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Titolo:
ELECTROROTATION OF ERYTHROCYTES TREATED WITH DIPICRYLAMINE - MOBILE CHARGES WITHIN THE MEMBRANE SHOW THEIR SIGNATURE IN ROTATIONAL SPECTRA
Autore:
SUKHORUKOV VL; ZIMMERMANN U;
Indirizzi:
UNIV WURZBURG,LEHRSTUHL BIOTECHNOL,AM HUBLAND D-97074 WURZBURG GERMANY UNIV WURZBURG,LEHRSTUHL BIOTECHNOL D-97074 WURZBURG GERMANY
Titolo Testata:
The Journal of membrane biology
fascicolo: 2, volume: 153, anno: 1996,
pagine: 161 - 169
SICI:
0022-2631(1996)153:2<161:EOETWD>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIPID BILAYER-MEMBRANES; SQUID AXON MEMBRANE; VALONIA-UTRICULARIS; ELECTRICAL-PROPERTIES; TRANSPORT KINETICS; PULSE RELAXATION; HYDROPHOBIC IONS; LIPOPHILIC IONS; PLASMA-MEMBRANE; MAMMALIAN-CELLS;
Keywords:
MOBILE CHARGES; DIELECTRIC SPECTROSCOPY; ELECTROROTATION; DISPERSION; MEMBRANE CAPACITY; MEMBRANE CONDUCTIVITY; LIPOPHILIC ION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
V.L. Sukhorukov e U. Zimmermann, "ELECTROROTATION OF ERYTHROCYTES TREATED WITH DIPICRYLAMINE - MOBILE CHARGES WITHIN THE MEMBRANE SHOW THEIR SIGNATURE IN ROTATIONAL SPECTRA", The Journal of membrane biology, 153(2), 1996, pp. 161-169

Abstract

In this study, electrorotation spectra of individual cells (that is, frequency dependence of cell rotation speed) have been proved to yieldinformation not only about the passive electric properties of cell constituents, but also about the presence of mobile charges within the plasma membrane being part of ion carrier transport systems. Experiments on human erythrocytes pretreated with the lipophilic anion dipicrylamine (DPA) gave convincing evidence that these artificial mobile charges adsorbed to the plasma membrane contributed significantly to the rotational spectrum at relatively low conductivity of the external medium (2-5 mS m(-1)). Theoretical integration of the mobile charge conceptinto the single-shell model (viewing the cell as a homogenous sphere surrounded by a membrane) led to a set of equations which predicted electrorotational behavior of DPA-treated cells in dependence on medium conductivity. The quantitative data on the partition and the transmembrane translocation rate of the DPA anion extracted from the experimental rotational spectra agreed well with the corresponding literature values.

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Documento generato il 17/01/21 alle ore 17:05:24