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Titolo:
IDENTIFICATION OF IQGAP AS A PUTATIVE TARGET FOR THE SMALL GTPASES, CDC42 AND RAC1
Autore:
KURODA S; FUKATA M; KOBAYASHI K; NAKAFUKU M; NOMURA N; IWAMATSU A; KAIBUCHI K;
Indirizzi:
NARA INST SCI & TECHNOL,DIV SIGNAL TRANSDUCT,8916-5 TAKAYAMA IKOMA 63001 JAPAN NARA INST SCI & TECHNOL,DIV SIGNAL TRANSDUCT IKOMA 63001 JAPAN HIROSHIMA UNIV,SCH MED,DEPT BIOCHEM,MINAMI KU HIROSHIMA 734 JAPAN KAZUSA DNA RES INST KISARAZU CHIBA 292 JAPAN KIRIN BREWERY CO LTD,CENT LABS KEY TECHNOL,KANAZAWA KU YOKOHAMA KANAGAWA 236 JAPAN
Titolo Testata:
The Journal of biological chemistry
fascicolo: 38, volume: 271, anno: 1996,
pagine: 23363 - 23367
SICI:
0021-9258(1996)271:38<23363:IOIAAP>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
BINDING PROTEIN RAC; GROWTH-FACTOR; PHOSPHOINOSITIDE 3-KINASE; KB CELLS; RHO P21; KINASE; INSULIN; ACTIVATION; DROSOPHILA; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
S. Kuroda et al., "IDENTIFICATION OF IQGAP AS A PUTATIVE TARGET FOR THE SMALL GTPASES, CDC42 AND RAC1", The Journal of biological chemistry, 271(38), 1996, pp. 23363-23367

Abstract

Cdc42 and Rac1 have been implicated in the regulation of various cellfunctions such as cell morphology, polarity, and cell proliferation. We have partially purified a Cdc42- and Rac1-associated protein with molecular mass of about 170 kDa (p170) from bovine brain cytosol. This protein interacted with guanosine 5'-(3-O-thio)triphosphate (GTP gammaS)-glutathione S-transferase (GST)-Cdc42 and GTP gamma-GST-Rac1, or GTP gamma S-GST-RhoA). We identified p170 as an IQGAP, which is originally identified as putative Ras GTPase-activating protein. Recombinant IQGAP specifically interacted with GTP gamma S-Cdc42 and GTP gamma S-Rac1. The C-terminal fragment of IQGAP was specifically immunoprecipitated with dominant-active Cdc42(Val12) or Rac1(Val12) from the COS7 cells expressing Cdc42(Val12) or Rac1(Val12), respectively, Immunofluorescence analysis revealed that IQGAP was accumulated at insulin- or Rac1-induced membrane ruffling areas. This accumulation of IQGAP was blocked by the microinjection of the dominant-negative Rac1(Asn17) or Cdc42(Asn17). Moreover, IQGAP was accumulated at the cell-cell junction in MDCK cells, where alpha-catenin and ZO-1 were localized. These resultssuggest that IQGAP is a novel target molecule for Cdc42 and Rac1.

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Documento generato il 18/01/21 alle ore 16:26:31