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Titolo:
CD14 IS A CELL-ACTIVATING RECEPTOR FOR BACTERIAL PEPTIDOGLYCAN
Autore:
GUPTA D; KIRKLAND TN; VIRIYAKOSOL S; DZIARSKI R;
Indirizzi:
INDIANA UNIV,SCH MED,NW CTR MED EDUC,3400 BROADWAY GARY IN 46408 INDIANA UNIV,SCH MED,NW CTR MED EDUC GARY IN 46408 UNIV CALIF SAN DIEGO,VET AFFAIRS MED CTR,DEPT MED SAN DIEGO CA 92161 UNIV CALIF SAN DIEGO,VET AFFAIRS MED CTR,DEPT PATHOL SAN DIEGO CA 92161
Titolo Testata:
The Journal of biological chemistry
fascicolo: 38, volume: 271, anno: 1996,
pagine: 23310 - 23316
SICI:
0021-9258(1996)271:38<23310:CIACRF>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
NF-KAPPA-B; LPS BINDING-PROTEIN; LIPOPOLYSACCHARIDE (LPS)-BINDING PROTEIN; NECROSIS-FACTOR-ALPHA; LIPID-A; STAPHYLOCOCCUS-AUREUS; SIGNAL-TRANSDUCTION; LIPOTEICHOIC ACID; HUMAN MONOCYTES; MACROPHAGES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
D. Gupta et al., "CD14 IS A CELL-ACTIVATING RECEPTOR FOR BACTERIAL PEPTIDOGLYCAN", The Journal of biological chemistry, 271(38), 1996, pp. 23310-23316

Abstract

The hypothesis that CD14 (an endotoxin receptor present on macrophages and neutrophils) acts as a cell-activating receptor for bacterial peptidoglycan was tested using mouse 70Z/3 cells transfected with human CD14. 70Z/3 cells transfected with an empty vector were unresponsive to insoluble and soluble peptidoglycan, as well as to low concentrations of endotoxin, 70Z/3-CD14 cells were responsive to both insoluble andsoluble peptidoglycan, as well as to low concentrations of endotoxin,as measured by the expression of surface IgM, activation of NF-kappa B, and degradation of I kappa B-alpha. Peptidoglycan also induced activation of NF-kappa B and degradation of I kappa B-alpha in macrophage RAW264.7 cells. These peptidoglycan-induced effects (in contrast to endotoxin-induced effects) were not inhibited by polymyxin B. Both peptidoglycan- and endotoxin-induced activation of NF-kappa B were inhibited by anti-CD14 mAb. The N-terminal 151 amino acids of CD14 were sufficient for acquisition of full responsiveness to both peptidoglycan and endotoxin, but CD14 deletion mutants lacking four small regions withinthe N-terminal 65 amino acids showed differentially diminished responses to peptidoglycan and endotoxin. These results identify CD14 as thefunctional receptor for peptidoglycan and demonstrate that similar, but not identical sequences in the N-terminal 65-amino acid region of CD14 are critical for the NF-kappa B and IgM responses to both peptidoglycan and endotoxin.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/12/18 alle ore 22:59:02