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Titolo:
THE USE OF IN-VITRO PREPARATIONS OF THE ISOLATED AMPHIBIAN CENTRAL-NERVOUS-SYSTEM IN NEUROANATOMY AND ELECTROPHYSIOLOGY
Autore:
LUKSCH H; WALKOWIAK W; MUNOZ A; TENDONKELAAR HJ;
Indirizzi:
CATHOLIC UNIV NIJMEGEN,DEPT ANAT & EMBRYOL,FAC MED SCI,POB 9101 NL-6500 HB NIJMEGEN NETHERLANDS CATHOLIC UNIV NIJMEGEN,DEPT ANAT & EMBRYOL,FAC MED SCI NL-6500 HB NIJMEGEN NETHERLANDS UNIV COLOGNE,INST ZOOL D-50923 COLOGNE GERMANY UNIV COMPLUTENSE MADRID,DEPT BIOL CELULAR,FAC CIENCIAS BIOL E-28040 MADRID SPAIN
Titolo Testata:
Journal of neuroscience methods
fascicolo: 1, volume: 70, anno: 1996,
pagine: 91 - 102
SICI:
0165-0270(1996)70:1<91:TUOIPO>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXCITATORY SYNAPTIC TRANSMISSION; MOTOR PATTERN GENERATION; SPINAL-CORD; DEXTRAN-AMINES; VISUAL-CORTEX; BRAIN-STEM; IN-VITRO; HORSERADISH-PEROXIDASE; MONODELPHIS-DOMESTICA; INTERNUCLEAR NEURONS;
Keywords:
AMPHIBIAN; ISOLATED BRAIN PREPARATION; TRACT-TRACING TECHNIQUE; INTRACELLULAR RECORDING; INTRACELLULAR STAINING; AUDITORY PATHWAY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
64
Recensione:
Indirizzi per estratti:
Citazione:
H. Luksch et al., "THE USE OF IN-VITRO PREPARATIONS OF THE ISOLATED AMPHIBIAN CENTRAL-NERVOUS-SYSTEM IN NEUROANATOMY AND ELECTROPHYSIOLOGY", Journal of neuroscience methods, 70(1), 1996, pp. 91-102

Abstract

In the present study an isolated preparation of the complete anuran central nervous system (CNS) is described which can be kept alive for several days and allows tracing, immunohistochemical and electrophysiological studies. A simple perfusion chamber is being used in which the isolated CNS preparation is superfused with oxygenated Ringer. The useof an isolated CNS has many advantages including: (1) virtually all areas are easily accessible at the same time without having the problemof blood vessels that hinder access; (2) large lesions and massive tracer applications are possible without survival problems of the animal, and tracers will not be translocated by blood circulation; (3) sincepulsations caused by the pressure chan es of blood circulation do notoccur, intracellular recordings are comparatively easy and stable; and (4) this approach offers the possibility of working on the same brain for several days by storing the preparation in a refrigerator overnight at low temperatures, thus allowing extensive utilization of a single preparation and reduction in the number of experimental animals required, Some applications to the anuran auditory system illustrate thatthe isolated anuran CNS is well-suited for a variety of neuroanatomical and physiological techniques.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/11/20 alle ore 21:36:34