Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
RETROVIRAL MISEXPRESSION OF ENGRAILED GENES IN THE CHICK OPTIC TECTUMPERTURBS THE TOPOGRAPHIC TARGETING OF RETINAL AXONS
Autore:
FRIEDMAN GC; OLEARY DDM;
Indirizzi:
SALK INST BIOL STUDIES,MOL NEUROBIOL LAB,10010 N TORREY PINES RD LA JOLLA CA 92037 SALK INST BIOL STUDIES,MOL NEUROBIOL LAB LA JOLLA CA 92037
Titolo Testata:
The Journal of neuroscience
fascicolo: 17, volume: 16, anno: 1996,
pagine: 5498 - 5509
SICI:
0270-6474(1996)16:17<5498:RMOEGI>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTOR TYROSINE KINASES; CLONALLY RELATED CELLS; PROTO-ONCOGENE INT-1; RETINOTECTAL PROJECTION; CEREBELLAR DEVELOPMENT; ROSTROCAUDAL POLARITY; EMBRYONIC EXPRESSION; VISUAL-SYSTEM; MOUSE-BRAIN; HOMEO BOX;
Keywords:
AXONAL GUIDANCE; GENE TRANSFER; HOMEOBOX; NEURAL MAPS; RECOMBINANT RETROVIRUS; RETINOTECTAL; TRANSCRIPTION FACTORS; VISUAL SYSTEM DEVELOPMENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
78
Recensione:
Indirizzi per estratti:
Citazione:
G.C. Friedman e D.D.M. Oleary, "RETROVIRAL MISEXPRESSION OF ENGRAILED GENES IN THE CHICK OPTIC TECTUMPERTURBS THE TOPOGRAPHIC TARGETING OF RETINAL AXONS", The Journal of neuroscience, 16(17), 1996, pp. 5498-5509

Abstract

We have investigated the role of the homeodomain transcription factorgenes En-1 and En-2, homologs of the Drosophila segment polarity geneengrailed, in regulating the development of the retinotopic map in the chick optic tectum. The En proteins are distributed in a gradient along the rostral-caudal axis of the developing tectum, with highest amounts found caudally. Previous evidence suggests that En-1 and En-2 mayregulate the polarity of the rostral-caudal axis of the tectum and the subsequent topographic mapping of retinal axons, We have tested thishypothesis by using a recombinant replication-competent retrovirus tooverexpress the En-1 or En-2 genes in the developing tectum. Anterograde labeling with the axon tracer Dir was used to analyze the topographic mapping of retinal axons after the lime that the retinotectal projection is normally topographically organized. Overexpression of eitherEn-1 or En-2 perturbed the topographic targeting of retinal axons. InEn-infected tecta, nasal retinal axons form an abnormally diffuse projection with numerous aberrant axons, branches, and arbors found at topographically incorrect locations, colocalized with domains of viral infection, In contrast, temporal axons did not form a diffuse projection or discrete aberrant arbors; however, many temporal axons were stunted and ended aberrantly rostral to their appropriate TZ, or in other cases either did not enter the tectum or formed a dense termination at its extreme rostral edge. These findings indicate that En-1 and En-2 are involved in regulating the development of the retinotopic map in the tectum. Furthermore, they support the hypothesis that En genes regulate the polarity of the rostral-caudal axis of the tectum, most likelyby controlling the expression of retinal axon guidance molecules.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 23:39:13