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Titolo:
THE ISOLATION AND CHARACTERIZATION OF A GENE ENCODING SUPEROXIDE-DISMUTASE FROM BRADYRHIZOBIUM SP (PARASPONIA) STRAIN ANU289
Autore:
THYGESEN PW; WHELAN J; MORELL MK; DAY DA;
Indirizzi:
AUSTRALIAN NATL UNIV,FAC SCI,DIV BIOCHEM & MOL BIOL CANBERRA ACT 0200AUSTRALIA AUSTRALIAN NATL UNIV,FAC SCI,DIV BIOCHEM & MOL BIOL CANBERRA ACT 0200AUSTRALIA AUSTRALIAN NATL UNIV,RES SCH BIOL SCI,DIRECTORS UNIT CANBERRA ACT 0200 AUSTRALIA
Titolo Testata:
Biochemistry and molecular biology international
fascicolo: 3, volume: 37, anno: 1995,
pagine: 401 - 412
SICI:
1039-9712(1995)37:3<401:TIACOA>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITROGEN-FIXATION; ACETYLENE-REDUCTION; SESBANIA-ROSTRATA; ESCHERICHIA-COLI; ROOT-NODULES; OXYGEN; RESPIRATION; BACTEROIDS; PROTEIN; PLANTS;
Keywords:
BRADYRHIZOBIUM; PARASPONIA; STRAIN ANU289; MN-SOD; SOD; SUPEROXIDE DISMUTASE; ESCHERICHIA-COLI QC779; SODA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
P.W. Thygesen et al., "THE ISOLATION AND CHARACTERIZATION OF A GENE ENCODING SUPEROXIDE-DISMUTASE FROM BRADYRHIZOBIUM SP (PARASPONIA) STRAIN ANU289", Biochemistry and molecular biology international, 37(3), 1995, pp. 401-412

Abstract

Bradyrhizobium sp. (Parasponia) strain ANU289 expresses a single Mn-SOD in both the vegetative and symbiotic states. A 500 bp sod-homologous sequence was amplified from genomic DNA of strain ANU289 using PCR. A 1.3 kb SalI fragment was subsequently cloned which contained an ORF,sodA, encoding a 23 Kd protein. This putative SOD shares considerablehomology with other Mn-SODs and analysis of the sodA sequence predicts that it is expressed. A lacZ-sodA fusion complemented the SOD-deficiency of E. coli QC779 and resulted in the expression of SOD activity in both mutant and wild type E. coli. We conclude that sodA encodes theMn-SOD of strain ANU289.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 22:00:28