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Titolo:
IDENTIFICATION OF MONOCLONAL-ANTIBODIES THAT RECOGNIZE DIFFERENT DISULFIDE-BONDED FORMS OF THROMBOSPONDIN-1
Autore:
MATTHIAS LJ; GOTISGRAHAM I; UNDERWOOD PA; MCNEIL HP; HOGG PJ;
Indirizzi:
UNIV NEW S WALES,CTR THROMBOSIS & VASC RES,SCH PATHOL SYDNEY NSW 2052AUSTRALIA UNIV NEW S WALES,CTR THROMBOSIS & VASC RES,SCH PATHOL SYDNEY NSW 2052AUSTRALIA UNIV NEW S WALES,INFLAMMAT RES UNIT,SCH PATHOL SYDNEY NSW 2052 AUSTRALIA CSIRO,DIV BIOMOL ENGN NORTH RYDE NSW 2113 AUSTRALIA
Titolo Testata:
Biochimica et biophysica acta. Protein structure and molecular enzymology
fascicolo: 2, volume: 1296, anno: 1996,
pagine: 138 - 144
SICI:
0167-4838(1996)1296:2<138:IOMTRD>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXTRACELLULAR-MATRIX; ACTIVATED PLATELETS; BINDING; INHIBITOR; PROTEIN; CELLS; LOCALIZATION;
Keywords:
GLYCOPROTEIN; MULTIDOMAIN; THROMBOSPONDIN 1; MONOCLONAL ANTIBODY; SULFHYDRYL-DISULFIDE INTERCHANGE; INFLAMMATION; CELL-MATRIX INTERACTION; (HUMAN RHEUMATOID SYNOVIAL FLUID);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
L.J. Matthias et al., "IDENTIFICATION OF MONOCLONAL-ANTIBODIES THAT RECOGNIZE DIFFERENT DISULFIDE-BONDED FORMS OF THROMBOSPONDIN-1", Biochimica et biophysica acta. Protein structure and molecular enzymology, 1296(2), 1996, pp. 138-144

Abstract

Thrombospondin 1 (TSP1) is a multidomain glycoprotein from platelets and cells which functions in cell-cell and cell-matrix interactions. The structure of TSP1 is regulated by sulfhydryl-disulfide interchange in the carboxy-terminal Ca2+-binding loops and globular domain which markedly influence its interaction with cell surface integrins and its inhibition of neutrophil enzymes. We have identified murine monoclonalantibodies that recognized different disulfide-bonded forms of TSP1, made by preparing TSP1 in buffers containing either 0.1 mM or 2 mM Ca2. Antibody HB8432 recognizes TSP1 prepared in buffers containing either 0.1 or 2 mM Ca2+, while antibodies D4.6 and A65M recognized only TSP1 prepared in buffers containing 0.1 mM Ca2+. The antibodies recognize these different TSP1 preparations either adsorbed to plastic or extracellular matrix. Immunohistochemistry of human rheumatoid synovial tissue using HB8432 resulted in staining of numerous blood vessel walls and matrix cells, while D4.6 and A65M stained a subset of the HB8432 positive blood vessels and only occasionally stained matrix cells. These results suggested that different disulfide-bonded forms of TSP1 werebeing expressed in different areas of inflamed tissue.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 03:11:26