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Titolo:
NMR ANALYSES OF COMPATIBLE SOLUTES IN A HALOTOLERANT BREVIBACTERIUM SP
Autore:
NAGATA S; ADACHI K; SANO H;
Indirizzi:
KOBE UNIV MERCANTILE MARINE,RES INST MARINE CARGO TRANSPORTAT,HIGASHINADA KU KOBE HYOGO 658 JAPAN MARINE BIOTECHNOL INST CO LTD SHIMIZU SHIZUOKA 424 JAPAN
Titolo Testata:
Microbiology
, volume: 142, anno: 1996,
parte:, 12
pagine: 3355 - 3362
SICI:
1350-0872(1996)142:<3355:NAOCSI>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI; HALOPHILIC EUBACTERIA; STRESS; ACCUMULATION; TETRAHYDROPYRIMIDINES; SPECTROSCOPY; ADAPTATION; SALT;
Keywords:
COMPATIBLE SOLUTES; HALOTOLERANCE; BREVIBACTERIUM SP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
20
Recensione:
Indirizzi per estratti:
Citazione:
S. Nagata et al., "NMR ANALYSES OF COMPATIBLE SOLUTES IN A HALOTOLERANT BREVIBACTERIUM SP", Microbiology, 142, 1996, pp. 3355-3362

Abstract

Internal solutes in a halotolerant Brevibacterium sp. (strain JCM 6894) were analysed by H-1 NMR spectroscopy. At the lower osmolarity of complex medium, the main compatible solutes in the cells were glutamate, glycine betaine and hydroxyproline, At higher external salt concentrations, ectoine 1,4,5,6-tetrahydro-2-methyl-4-pyrimidinecarboxylic acid) levels increased and this became the predominant compatible solute at > 1.5 M NaCl or KCl. When Brevibacterium sp. was grown in the absence of yeast extract, glycine betaine levels were reduced to about halfand a new solute, glycine, was detected. Irrespective of whether the medium contained yeast extract, Brevibacterium sp. accumulated hydroxyproline, which has not been previously reported as an osmolyte in halophilic eubacteria, Trehalose and glutamate were the main solutes in cells grown in chemically defined medium containing 0-0.5 M NaCl. When grown in the presence of > 1.5 M NaCl, both ectoine and hydroxyectoine became dominant, as was also the case in complex medium. Additional compatible solutes detected were gamma-aminobutyrate and proline, neither of which were detected in cells grown in complex medium. Regardless of the medium composition and incubation time, ectoine levels were high at high osmolarity, indicating that the halotolerant nature of Brevibacterium sp. is closely related to its accumulation of ectoine. From its accumulation of compatible solutes, we suggest that Brevibacteriumsp, JCM 6894 is a different species from other members of the genus Brevibacterium.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 04:14:14