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Titolo:
DIFFERENCES IN V-KAPPA GENE UTILIZATION AND VH CDR3 SEQUENCE AMONG ANTI-DNA FROM C3H-LPR MICE AND LUPUS MICE WITH NEPHRITIS
Autore:
WLOCH MK; ALEXANDER AL; PIPPEN AMM; PISETSKY DS; GILKESON GS;
Indirizzi:
VET ADM MED CTR,508 FULTON,BOX 151G DURHAM NC 27705 DUKE UNIV,MED CTR,DEPT MED DURHAM NC 27710 DUKE UNIV,MED CTR,DEPT IMMUNOL DURHAM NC 27710 DURHAM VET ADM MED RES SERV DURHAM NC 00000
Titolo Testata:
European Journal of Immunology
fascicolo: 9, volume: 26, anno: 1996,
pagine: 2225 - 2233
SICI:
0014-2980(1996)26:9<2225:DIVGUA>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLOMERULAR BASEMENT-MEMBRANE; MRL-LPR MICE; MOLECULAR CHARACTERIZATION; MONOCLONAL-ANTIBODIES; VARIABLE REGIONS; MOUSE STRAINS; RENAL-DISEASE; B-CELLS; NEPHRITOGENIC AUTOANTIBODIES; STRUCTURAL CHARACTERISTICS;
Keywords:
ANTI-DNA; C3H-LPR; V GENE USAGE; VH CDR3 SEQUENCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
89
Recensione:
Indirizzi per estratti:
Citazione:
M.K. Wloch et al., "DIFFERENCES IN V-KAPPA GENE UTILIZATION AND VH CDR3 SEQUENCE AMONG ANTI-DNA FROM C3H-LPR MICE AND LUPUS MICE WITH NEPHRITIS", European Journal of Immunology, 26(9), 1996, pp. 2225-2233

Abstract

To investigate the molecular properties of anti-DNA from lpr mice that express high levels of anti-DNA without immune-mediated nephritis, the sequences of VH and V kappa genes encoding 11 monoclonal anti-DNA antibodies derived from C3H-lpr/lpr (C3H-lpr) mice were studied. All ofthe C3H-lpr monoclonal anti-DNA bound single-stranded DNA while five also bound double-stranded DNA. Two of the hybridomas were clonally related as determined by Southern analysis and sequencing. Sequence analysis of C3H-lpr anti-DNA revealed the use of VH genes that encode anti-DNA from the MRL-lpr/lpr and (NZB X NZW)F1 mouse models of lupus, although differences occurred in the VH CDR3 amino acid content. In contrast, the Vr. genes from C3H-lpr mice lacked significant identity with previously reported Vx genes for anti-DNA from lupus models. These results indicate that anti-DNA from C3H-lpr mice differ from anti-DNA from lupus mice with nephritis in patterns of V gene expression and suggest a molecular basis for the lack of pathogenicity of anti-DNA in these mice.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 13:47:29