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Titolo:
MYOSIN HEAVY-CHAIN MESSENGER-RNA AND PROTEIN EXPRESSION IN SINGLE FIBERS OF THE RAT SOLEUS FOLLOWING REINNERVATION
Autore:
BODINE SC; PIEROTTI DJ;
Indirizzi:
REGENERON PHARMACEUT INC,777 OLD SAW MILL RIVER RD TARRYTOWN NY 10591 UNIV CALIF SAN DIEGO,SCH MED,PROGRAM BIOMED SCI,VA MED CTR LA JOLLA CA 92093
Titolo Testata:
Neuroscience letters
fascicolo: 1, volume: 215, anno: 1996,
pagine: 13 - 16
SICI:
0304-3940(1996)215:1<13:MHMAPE>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
SKELETAL-MUSCLE; ISOFORMS; SLOW; GENE;
Keywords:
DENERVATION; SCIATIC NERVE INJURY; IN SITU HISTOCHEMISTRY; IMMUNOHISTOCHEMISTRY; MYOSIN HEAVY CHAIN GENE REGULATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
S.C. Bodine e D.J. Pierotti, "MYOSIN HEAVY-CHAIN MESSENGER-RNA AND PROTEIN EXPRESSION IN SINGLE FIBERS OF THE RAT SOLEUS FOLLOWING REINNERVATION", Neuroscience letters, 215(1), 1996, pp. 13-16

Abstract

Myosin heavy chain (MHC) expression is regulated by many factors including neural input. To gain a better understanding of myosin transformation following reinnervation we examined both MHC protein and mRNA insingle fibers of the soleus. A midthigh sciatic nerve lesion resultedin reinnervation of the soleus by motoneurons from both the original and foreign motor pools. MHC expression was examined in individual fibers 8 and 16 weeks post injury using in situ histochemistry and immunohistochemistry. Following a sciatic nerve lesion, the reinnervated soleus underwent a transformation from slow toward fast based on physiologic and biochemical measurements, At 8 weeks, fast MHC mRNA isoforms (IIa and IIx) were upregulated and slow mRNA was downregulated, however, the predominant protein isoform was MHC I. At both 8 and 16 weeks, many fibers expressed multiple mRNA isoforms. At 16 weeks there was limited co-expression of slow and fast MHC mRNAs, but continued co-expression of fast MHC mRNAs. Sixteen weeks following reinnervation the predominant fast mRNA and protein in the soleus was IIx MHC.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 04:55:47