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Titolo:
E2F BINDING IS REQUIRED BUT NOT SUFFICIENT FOR REPRESSION OF B-MYB TRANSCRIPTION IN QUIESCENT FIBROBLASTS
Autore:
BENNETT JD; FARLIE PG; WATSON RJ;
Indirizzi:
LUDWIG INST CANC RES,IMPERIAL COLL,SCH MED ST MARYS,NORFOLK PL LONDONW2 1PG ENGLAND LUDWIG INST CANC RES,IMPERIAL COLL,SCH MED ST MARYS LONDON W2 1PG ENGLAND
Titolo Testata:
Oncogene
fascicolo: 5, volume: 13, anno: 1996,
pagine: 1073 - 1082
SICI:
0950-9232(1996)13:5<1073:EBIRBN>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-CYCLE PROGRESSION; RETINOBLASTOMA PROTEIN; GROWTH-REGULATION; S-PHASE; IN-VIVO; E2F-CYCLIN-A COMPLEX; FAMILY PROTEINS; GENE PROMOTER; EXPRESSION; ASSOCIATION;
Keywords:
B-MYB; E2F; CELL CYCLE; TRANSCRIPTION REPRESSION; P107; P130;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
52
Recensione:
Indirizzi per estratti:
Citazione:
J.D. Bennett et al., "E2F BINDING IS REQUIRED BUT NOT SUFFICIENT FOR REPRESSION OF B-MYB TRANSCRIPTION IN QUIESCENT FIBROBLASTS", Oncogene, 13(5), 1996, pp. 1073-1082

Abstract

We have previously shown in mouse NIH3T3 fibroblasts that transcription of the B-myb gene, which encodes a transcription factor required for S phase entry, is repressed through a promoter E2F site in G(0)/early G(1). Transcription repression at this stage of the cell cycle was correlated with binding of a specific p107/E2F complex to this site, Wereport here, however, that transfection of cells with the known components of this complex, p107, E2F-4 and DP-1, did not repress the B-mybpromoter in cycling NIH3T3 cells, although p107 inhibited transcription transactivation by E2F-4/DP-1. To establish definitively the contribution of E2F to repression, the effects of further mutations within and surrounding the E2F site were examined, It was evident that E2F binding and repression were closely correlated, lending greater weight tothe contention that E2F itself is implicated in this activity, These studies also identified a closely linked site, designated the downstream repression site (DRS), which was not required for E2F binding or transactivation but which was necessary for repression, These findings indicated that E2F-dependent repression and activation are independently regulated phenomena and suggest that repression involves additional interactions determined by the promoter context.

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Documento generato il 27/09/20 alle ore 17:32:11