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Titolo:
BLOOD-GAS PARAMETERS AND THE RESPONSES OF ERYTHROCYTES IN CARP EXPOSED TO DEEP HYPOXIA AND SUBSEQUENT RECOVERY
Autore:
VANRAAIJ MTM; VIANEN GJ; VANDENTHILLART GEEJM;
Indirizzi:
LEIDEN UNIV,GORLAEUS LABS,DEPT BIOL,POB 9502 NL-2300 RA LEIDEN NETHERLANDS LEIDEN UNIV,GORLAEUS LABS,DEPT BIOL NL-2300 RA LEIDEN NETHERLANDS
Titolo Testata:
Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology
fascicolo: 7, volume: 166, anno: 1996,
pagine: 453 - 460
SICI:
0174-1578(1996)166:7<453:BPATRO>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
RED-CELL FUNCTION; ACID-BASE STATUS; CYPRINUS-CARPIO; RAINBOW-TROUT; OXYGEN-AFFINITY; ADRENERGIC RESPONSES; EXERCISE-STRESS; SALMO-GAIRDNERI; NA+/H+ EXCHANGE; CARBON-DIOXIDE;
Keywords:
CATECHOLAMINES; RED CELLS ERYTHROCYTES; ACID-BASE BALANCE; HYPOXIA; CARP, CYPRINUS CARPIO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
M.T.M. Vanraaij et al., "BLOOD-GAS PARAMETERS AND THE RESPONSES OF ERYTHROCYTES IN CARP EXPOSED TO DEEP HYPOXIA AND SUBSEQUENT RECOVERY", Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology, 166(7), 1996, pp. 453-460

Abstract

The quantitative importance of the adrenergic response of carp erythrocytes during severe oxygen restriction is not clear at present. Quantitative differences between in vivo and in vitro studies suggest that the response of carp erythrocytes may be dependent on the actual hypoxic condition. To our knowledge, a clear picture of the blood gas status, erythrocytic responses and catecholamines measured simultaneously in carp exposed to deep severe hypoxia or anoxia has not yet been reported. Therefore, we studied the physiological response of carp exposed to deep hypoxia at 0.3 kPa and subsequent recovery. Carp were fitted with an indwelling cannula in the dorsal aorta for repeated blood sampling and the blood was analysed for hematocrit, hemoglobin, mean cellular hemoglobin content, intra- and extracellular pH, pO(2), pCO(2), total CO2 and catecholamines. Large fluctuations in arterial pO(2) levelswere observed in normoxic control carp, probably caused by the alternating breathing pattern of carp. Even at water pO(2) levels of 0.3 kPa, arterial pO(2) levels were maintained at about 0.2-0.3 kPa. Catecholamine levels were increased during deep hypoxia with noradrenaline as the predominant catecholamine. Hematological variables showed that thenumber of circulating erythrocytes was increased during hypoxia. The intracellular pH of carp red cells was maintained at pre-exposure values despite a considerable decrease of pH(e). In this in vivo study, a marked decrease of the proton gradient across the red cell membrane (pH(e)-pH(i)), as high as 0.35 pH units, was observed, which is quantitatively similar to that usually observed in salmonids during hypoxia. It is suggested that the regulation of the carp erythrocytic pH(i) is probably caused to a major extent by deoxygenation of hemoglobin (Haldane effect) while adrenergic activation of the red cells is likely to contribute significantly to the observed reduction of the proton gradient. These mechanisms result in the persistence of a capacity for aerobic metabolism in carp of about 10-20% of the energy metabolism despiteenvironmental pO(2) values of 2-3 mm Hg.

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Documento generato il 30/09/20 alle ore 12:44:18