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Titolo:
B-CELL EPITOPE MAPPING OF THE BACTERIAL SUPERANTIGEN STAPHYLOCOCCAL-ENTEROTOXIN-B - THE DOMINANT EPITOPE REGION RECOGNIZED BY INTRAVENOUS IGG
Autore:
NISHI JI; KANEKURA S; TAKEI S; KITAJIMA I; NAKAJIMA T; WAHID MR; MASUDA K; YOSHINAGA M; MARUYAMA I; MIYATA K;
Indirizzi:
KAGOSHIMA UNIV,FAC MED,DEPT PEDIAT,8-35-1 SAKURA GAOKA KAGOSHIMA 890 JAPAN KAGOSHIMA UNIV,FAC MED,DEPT LAB MOL MED KAGOSHIMA 890 JAPAN
Titolo Testata:
The Journal of immunology
fascicolo: 1, volume: 158, anno: 1997,
pagine: 247 - 254
SICI:
0022-1767(1997)158:1<247:BEMOTB>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
TOXIC SHOCK SYNDROME; T-CELLS; KAWASAKI-DISEASE; YERSINIA-PSEUDOTUBERCULOSIS; RHEUMATOID-ARTHRITIS; SELECTIVE EXPANSION; POLYPEPTIDE HORMONE; THYMOPOIETIN; THYMOPENTIN; SEQUENCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
J.I. Nishi et al., "B-CELL EPITOPE MAPPING OF THE BACTERIAL SUPERANTIGEN STAPHYLOCOCCAL-ENTEROTOXIN-B - THE DOMINANT EPITOPE REGION RECOGNIZED BY INTRAVENOUS IGG", The Journal of immunology, 158(1), 1997, pp. 247-254

Abstract

We showed that i.v. IgG contains Abs against a major group of bacterial superantigens, and that they can inhibit superantigen-elicited T cell activation, The B cell epitope region of the superantigen and the inhibitory mechanism have remained unknown. To analyze the dominant B cell epitopes on the bacterial superantigen SEE (staphylococcal enterotoxin B), we constructed fusion proteins of SEE deletion mutants, and the reactivities of these recombinant proteins to i.v. IgG and healthy human sera were evaluated by means of immunoblotting, Intravenous IgG and healthy human sera mostly recognized the C-terminal fragment (amino acid (aa) 133-239). The C-terminally truncated protein (aa 1-228) and the truncated mutant Delta 225-234 lost reactivity, while the truncated protein (aa 1-234) did not, suggesting that the region (aa 225-234) is the dominant B cell epitope. The mutant, in which residues 226-229 of SEB were exchanged for residues 209-212 of streptococcal pyrogenic exotoxin A, reduced the reactivity with the C-terminal region-specific IgG purified by affinity chromatography, The C-terminal region-specific IgG inhibited SEB-elicited T cell activation, suggesting that this Ab that recognizes the epitope functions as the humoral defensive factor against SEE in humans, Furthermore, the assumed epitope region was homology to the residues (aa 32-41) of human thymopoietin, containing the biologic active site.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 03:38:55