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Titolo:
DNA ANALYSIS-METHODS FOR RECOGNIZING SPECIES INVASION - THE EXAMPLE OF CODIUM, AND GENERALLY APPLICABLE METHODS FOR ALGAE
Autore:
COLEMAN AW;
Indirizzi:
BROWN UNIV,DIV BIOL & MED PROVIDENCE RI 02912
Titolo Testata:
Hydrobiologia
, volume: 327, anno: 1996,
pagine: 29 - 34
SICI:
0018-8158(1996)327:<29:DAFRSI>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERNAL TRANSCRIBED SPACER; CHLOROCOCCALEAN GREEN-ALGAE; DIATOM SKELETONEMA-COSTATUM; CHLOROPLAST DNA; MOLECULAR DELINEATION; GRACILARIA-VERRUCOSA; SEQUENCE DATA; 18S RDNA; RHODOPHYTA; CHLOROPHYTA;
Keywords:
CODIUM; INTRODUCED SPECIES; RDNA; ITS; RFLPS; SEAWEED;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
A.W. Coleman, "DNA ANALYSIS-METHODS FOR RECOGNIZING SPECIES INVASION - THE EXAMPLE OF CODIUM, AND GENERALLY APPLICABLE METHODS FOR ALGAE", Hydrobiologia, 327, 1996, pp. 29-34

Abstract

Analysis of DNA can help to distinguish those morphological characters indicative of species difference from those representing retained traits or parallel evolution. This can be of great value in detecting recent invaders. The choice of which DNA characters to examine not only dictates the methodology to be used but must also be appropriate for the detection level sought. Restriction endonuclease fragment comparisons of plastid DNA have been used to assess Codium species; the resultsshow C. fragile subsp. tomentosoides from east and west coast North America to be identical while sympatric endemic Codium species each display their own unique set of fragments. For species of other algae, plastid DNA fragment patterns are not necessarily identical across a morphological species, e.g. Pandorina morum. Such repetitive element probes as M13 and the use of RAPDs are more appropriate for analysis of populations within species. DNA base sequence comparisons of nuclear rDNA genes often yield too few variant bases between closely related species for reliable identifications. Analysis of the more variable Internal Transcribed Spacer (ITS) region, lying between the small and large ribosomal subunit genes in nuclear DNA, yields more extensive base pair variation between species and relatively little within species; it may be an alternative choice for endonuclease restriction fragment analysis or for sequencing.

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Documento generato il 20/09/20 alle ore 10:38:31