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Titolo:
EFFECTS OF STREPTOMYCIN AND AMILORIDE ON THE FUNCTION OF THE PIGEON INNER-EAR
Autore:
RICHTER CP; HEYNERT S; KLINKE R;
Indirizzi:
NORTHWESTERN UNIV,FRANCES SEARLE BLDG,2299 N CAMPUS DR EVANSTON IL 60208 UNIV FRANKFURT,ZENTRUM PHYSIOL D-60590 FRANKFURT GERMANY
Titolo Testata:
Journal of comparative physiology. A, Sensory, neural, and behavioral physiology
fascicolo: 3, volume: 179, anno: 1996,
pagine: 415 - 427
SICI:
0340-7594(1996)179:3<415:EOSAAO>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
COCHLEAR HAIR-CELLS; SINGLE-FIBER ACTIVITY; MECHANOELECTRICAL TRANSDUCTION; AMINOGLYCOSIDE ANTIBIOTICS; MEMBRANE-VESICLES; SODIUM-TRANSPORT; IONIC CURRENTS; CHANNELS; CHICK; ANALOGS;
Keywords:
BIRD; STREPTOMYCIN; AMILORIDE; INNER EAR; MECHANOELECTRICAL TRANSDUCTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
C.P. Richter et al., "EFFECTS OF STREPTOMYCIN AND AMILORIDE ON THE FUNCTION OF THE PIGEON INNER-EAR", Journal of comparative physiology. A, Sensory, neural, and behavioral physiology, 179(3), 1996, pp. 415-427

Abstract

In vitro, streptomycin and amiloride block the mechano-electrical transduction channels in isolated auditory hair cells of mammals and birds. The present in vivo experiments investigate their action in the intact pigeon inner ear. Streptomycin dissolved in artificial endolymph at concentrations between 1.0 and 6.2 mmol/l was applied into the scalamedia. After a bolus injection (0.09 to 0.36 mu l) the endocochlear potential (EP) was elevated from 6.8 to 7.9 mV. Similarly applied boli of 0.09 to 0.55 mu l of amiloride in concentrations of 1-2 mmol/l did not significantly elevate EP (8.7 versus 9.4 mV). Compound action potential (CAP) frequency threshold curves (FTCs) were elevated by both streptomycin and amiloride. The threshold elevation increased with frequency. With streptomycin injection, a complete loss of CAPs was found at frequencies above 1.2 kHz. With amiloride, the loss in sensitivity was 40 dB for the same frequency range. Single fibre recordings showed elevated thresholds and decreased sharpness of tuning with both drugs. With streptomycin, the evoked activity was affected regardless of stimulation frequencies (above or below the fibre's characteristic frequency). Amiloride, however, preferentially elevated thresholds at frequencies above characteristic frequency. Thus amiloride seems to interfere with hair cell tuning mechanisms. Spontaneous activity decreased by about 30%, following the application of streptomycin. In contrast, injections of amiloride into the scala media were followed by an increasein spontaneous discharge rates. When the drugs were applied into scala tympani, streptomycin reduced CAP amplitudes within 2 min. Amilorideproduced no effect if applied into the scala tympani.

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Documento generato il 25/11/20 alle ore 06:56:06