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Titolo:
PRODUCTION OF MONOCYTE CHEMOTACTIC PROTEIN-1 BY RAT-BRAIN MACROPHAGES
Autore:
CALVO CF; YOSHIMURA T; GELMAN M; MALLAT M;
Indirizzi:
COLL FRANCE,INSERM,CHAIRE NEUROPHARMACOL,U114 F-75231 PARIS 05 FRANCE NCI,IMMUNOPATHOL SECT,IMMUNOBIOL LAB FREDERICK MD 00000
Titolo Testata:
European journal of neuroscience
fascicolo: 8, volume: 8, anno: 1996,
pagine: 1725 - 1734
SICI:
0953-816X(1996)8:8<1725:POMCPB>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS; BLOOD MONONUCLEAR LEUKOCYTES; COLONY-STIMULATING FACTOR; AMINO-ACID ANALYSIS; COMPETENCE GENE-JE; CHEMOATTRACTANT PROTEIN-1; MOLECULAR-CLONING; GROWTH-FACTOR; CDNA CLONING;
Keywords:
MICROGLIA; CHEMOTACTIC MIGRATION; INFLAMMATORY CYTOKINES; BRAIN INJURY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
52
Recensione:
Indirizzi per estratti:
Citazione:
C.F. Calvo et al., "PRODUCTION OF MONOCYTE CHEMOTACTIC PROTEIN-1 BY RAT-BRAIN MACROPHAGES", European journal of neuroscience, 8(8), 1996, pp. 1725-1734

Abstract

In the present study, we show that cultured rat brain macrophages release a soluble factor that stimulates the migration of bone marrow-derived macrophages, as determined by an in vitro chemotaxis assay. A checkerboard analysis indicated that most of this effect resulted from a polarized migration of the cells (chemotactic phenomenon), rather thanin an increase in cell motility (chemokinesis). This activity was significantly decreased by an immune serum directed against the rat monocyte chemoattractant protein-1 (chemokine MCP-1). Northern blot analysis demonstrated expression of the MCP-1 gene in cultured brain macrophages, but its absence in unstimulated bone marrow-derived macrophages. Up-regulation of MCP-1 expression was observed when lipopolysaccharidewas added to cultured brain macrophages, a peak occurring after a 6 hperiod of stimulation. Also, inflammatory cytokines such as interleukin (II)-1 beta, colony stimulating factor-1, tumour necrosis factor-alpha and IL-6 individually increased the basal level of MCP-1 mRNA. Subsequently, we demonstrated the in vivo production of MCP-1 in the adult rat brain following injury induced by a local injection of kainic acid. MCP-1 synthesis was localized in both astrocytes and brain macrophages. These results suggest that the activation of resting microglial cells into brain macrophages and their subsequent secretion of chemokines could contribute to the mechanism(s), leading to the infiltration of the CNS by blood-derived monocytes, as observed in several pathologies.

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Documento generato il 24/09/20 alle ore 22:55:41