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Titolo:
ALTERED SUBCELLULAR-DISTRIBUTION OF U3 SNRNA IN RESPONSE TO SERUM IN MOUSE FIBROBLASTS
Autore:
SIENNA N; LARSON DE; SELLS BH;
Indirizzi:
UNIV GUELPH,DEPT MOL BIOL & GENET GUELPH ON N1G 2W1 CANADA UNIV GUELPH,DEPT MOL BIOL & GENET GUELPH ON N1G 2W1 CANADA
Titolo Testata:
Experimental cell research
fascicolo: 1, volume: 227, anno: 1996,
pagine: 98 - 105
SICI:
0014-4827(1996)227:1<98:ASOUSI>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRE-RIBOSOMAL-RNA; EXTERNAL TRANSCRIBED SPACER; SMALL NUCLEOLAR RNA; MYOBLAST DIFFERENTIATION; TRANSCRIPTIONAL REGULATION; PRERIBOSOMAL RNA; FIBRILLARIN; PROTEIN; YEAST; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
N. Sienna et al., "ALTERED SUBCELLULAR-DISTRIBUTION OF U3 SNRNA IN RESPONSE TO SERUM IN MOUSE FIBROBLASTS", Experimental cell research, 227(1), 1996, pp. 98-105

Abstract

To extend our understanding of the mechanisms regulating ribosome biosynthesis during changes in cellular growth rate, the expression and subcellular distribution of U3 snRNA and one of its associated proteins, fibrillarin, were examined in mouse 3T6 fibroblasts, Altering serum concentrations produces changes in the ribosome content of the cell asreflected by total RNA levels. When exponentially growing 3T6 cells are induced to become quiescent by serum starvation, a significant downshift in U3 snRNA gene transcription occurs in parallel to a decrease in pre-rRNA synthesis. Serum stimulation results in an increase in therate of synthesis of both U3 snRNA and pre-rRNA. However, U3 snRNA synthesis lags behind that of pre-rRNA, Furthermore, in serum-starved fibroblasts, a significant portion of the total cellular U3 snRNA appears in the cytoplasm. Following serum stimulation, a redistribution occurs and U3 snRNA is localized predominantly in the nucleolus at a levelsimilar to that observed in exponentially growing cells. This redistribution is inhibited when RNA or protein synthesis is repressed in serum-stimulated fibroblasts by actinomycin D or cycloheximide. In contrast, the level and subcellular distribution of fibrillarin remain unchanged during serum starvation, These results suggest that during changes in ribosome production, distinct pools of U3 snRNPs exist within thecell. (C) 1996 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 18:02:43