Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
FACILITATION OF APOPTOSIS BY AUTOLOGOUS SERUM AND RELATED IMMUNOSUPPRESSION IN THE SPLENOCYTE CULTURE
Autore:
KIM HM; OH GT; HONG DH; HYUN BH; CHA YN; YOO BS; HAN SB;
Indirizzi:
KIST,KOREA RES INST BIOSCI & BIOTECHNOL,POB 115 TAEJON 305600 SOUTH KOREA INHA UNIV,COLL MED INCHON SOUTH KOREA KYONGGI UNIV,COLL NAT SCI SUWON SOUTH KOREA
Titolo Testata:
Immunopharmacology
fascicolo: 1, volume: 34, anno: 1996,
pagine: 39 - 50
SICI:
0162-3109(1996)34:1<39:FOABAS>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROGRAMMED CELL-DEATH; NEGATIVE SELECTION; DNA FRAGMENTATION; T-CELLS; THYMOCYTES; LYMPHOCYTES; INDUCTION; DYSFUNCTION; HYPOTHESIS; ACTIVATION;
Keywords:
MOUSE SERUM; APOPTOSIS; DNA FRAGMENTATION; SPLENOCYTE; APOPTOTIC FACTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
H.M. Kim et al., "FACILITATION OF APOPTOSIS BY AUTOLOGOUS SERUM AND RELATED IMMUNOSUPPRESSION IN THE SPLENOCYTE CULTURE", Immunopharmacology, 34(1), 1996, pp. 39-50

Abstract

The addition of adult mouse serum (MS) to the culture of mouse splenocytes resulted in an accelerated decrease of viable cell number duringinitial 24 h of culture as determined by the trypan blue dye exclusion test and the propidium iodide staining method. Furthermore, the extent of DNA fragmentation, the hallmark of apoptosis, determined by agarose gel electrophoresis and the amount of fragmented DNA measured by ELISA method showed that the extent of apoptosis was clearly increased in splenocytes cultured in the presence of MS. Under the scanning and transmission electron microscopic observations, the large portion of splenocytes showed morphological characteristics of apoptotic cells such as apoptotic body, condensed chromatin and shrunken appearance. Withthe accelerated rate of apoptosis, the immunocompetence of splenocytes such as the antibody production, natural killer cell activity, and proliferation by mitogens was strongly suppressed. When analyzed by surface immunolabelling flow cytometry, the subsets of lymphocytes (B, T,CD4 + T and CD8 + T cells) were affected in a global non-selective manner. As determined by ultrafiltration, the molecular weights of apoptosis-facilitating factors present in MS appeared to be greater than 10kDa. Upon fractionation with Sephadex G-200, the apoptotic factors were separated into 2 fractions. In summary, results obtained in the present study indicate that some unidentified endogeneous macromolecules present in MS may produce the stimulatory effect on the apoptosis and cause immunosuppression of splenocytes under culture.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 07:21:04