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Titolo:
COORDINATED REGULATION OF HYALURONAN AND AGGRECAN CONTENT IN THE ARTICULAR-CARTILAGE OF IMMOBILIZED AND EXERCISED DOGS
Autore:
HAAPALA J; LAMMI MJ; INKINEN R; PARKKINEN JJ; AGREN UM; AROKOSKI J; KIVIRANTA I; HELMINEN HJ; TAMMI MI;
Indirizzi:
UNIV KUOPIO,DEPT ANAT,PL 1627 KUOPIO 70211 FINLAND UNIV KUOPIO,DEPT PATHOL KUOPIO 70211 FINLAND UNIV KUOPIO,DEPT SURG KUOPIO 70211 FINLAND KUOPIO UNIV HOSP,DEPT PHYS & REHABIL MED KUOPIO FINLAND
Titolo Testata:
Journal of rheumatology
fascicolo: 9, volume: 23, anno: 1996,
pagine: 1586 - 1593
SICI:
0315-162X(1996)23:9<1586:CROHAA>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXPERIMENTAL OSTEO-ARTHRITIS; KNEE STIFLE JOINT; PROTEOGLYCAN AGGREGATION; BIOCHEMICAL-CHANGES; RUNNING EXERCISE; BEAGLE DOGS; ACID; OSTEOARTHROSIS; REMOBILIZATION; IDENTIFICATION;
Keywords:
HYALURONAN; IMMOBILIZATION; JOINT LOADING; EXERCISE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
J. Haapala et al., "COORDINATED REGULATION OF HYALURONAN AND AGGRECAN CONTENT IN THE ARTICULAR-CARTILAGE OF IMMOBILIZED AND EXERCISED DOGS", Journal of rheumatology, 23(9), 1996, pp. 1586-1593

Abstract

Objective. To study the influence of joint loading and immobilizationon articular cartilage hyaluronan concentration and histological distribution in the knee joints: of young dogs subjected to II weeks' immobilization by splinting, and 15 weeks' running exercise at a rate of 40 km/day. Methods. The amount of hyaluronan in articular cartilage wasdetermined by a competitive binding assay using a biotinylated hyaluronan binding complex (HABC) of aggrecan and link protein. Histologic sections were stained for the localization of hyaluronan with the HABC probe. Extracted proteoglycans were characterized by sodium dodecyl sulfate agarose gel electrophoresis. Results. Immobilization significantly reduced the concentration of hyaluronan in all sites studied (tibial and femoral condyles, patellar surface of femur). The proportion of hyaluronan to total uronic acid (mainly from aggrecan) remained unchanged because of a concurrent decrease in aggrecan. The ratio of hyaluronan and aggrecan remained constant also in runners. The staining pattern of free hyaluronan in the tissue sections and the electrophoretic mobility of the extracted proteoglycans were not affected by the different loading regimes. Conclusion. Reduced joint loading due to splint immobilization significantly decreases both hyaluronan and aggrecan in the articular cartilage. The remarkably parallel changes in aggrecan and hyaluronan content suggest that joint loading exerts a coordinated influence on their metabolism.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 11:49:56