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Titolo:
THE YEAST GAL11 PROTEIN BINDS TO THE TRANSCRIPTION FACTOR IIE THROUGHGAL11 REGIONS ESSENTIAL FOR ITS IN-VIVO FUNCTION
Autore:
SAKURAI H; KIM YJ; OHISHI T; KORNBERG RD; FUKASAWA T;
Indirizzi:
KAZUSA DNA RES INST,1532-3 YANAUCHINO KISARAZU CHIBA 292 JAPAN KAZUSA DNA RES INST KISARAZU CHIBA 292 JAPAN KEIO UNIV,SCH MED,GENET MOL LAB,SHINJUKU KU TOKYO 160 JAPAN STANFORD UNIV,SCH MED,DEPT CELL BIOL STANFORD CA 94305
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 18, volume: 93, anno: 1996,
pagine: 9488 - 9492
SICI:
0027-8424(1996)93:18<9488:TYGPBT>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
RNA-POLYMERASE-II; SACCHAROMYCES-CEREVISIAE; BASAL TRANSCRIPTION; PROMOTER CLEARANCE; HOLOENZYME; GENE; INITIATION; DNA; EXPRESSION; ACTIVATORS;
Keywords:
SACCHAROMYCES CEREVISIAE; RNA POLYMERASE II HOLOENZYME;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
H. Sakurai et al., "THE YEAST GAL11 PROTEIN BINDS TO THE TRANSCRIPTION FACTOR IIE THROUGHGAL11 REGIONS ESSENTIAL FOR ITS IN-VIVO FUNCTION", Proceedings of the National Academy of Sciences of the United Statesof America, 93(18), 1996, pp. 9488-9492

Abstract

The GAL11 gene encodes an auxiliary transcription factor required forfull expression of many genes in yeast, The GAL11-encoded protein (Gal11p) has recently been shown to copurify with the holoenzyme of RNA polymerase II. Here we report that Gal11p stimulates basal transcription in a reconstituted transcription system composed of recombinant or highly purified transcription factors, TFIIB, TFIIE, TFIIF, TFIIH, and TATA box-binding protein and core RNA polymerase II, We further demonstrate that each of the two domains of Gal11p essential for in vivo function respectively participates in the binding to the small and large subunits of TFIIE. The largest subunit of RNA polymerase II was coprecipitated by anti-hemagglutinin epitope antibody from crude extract of GAL11 wild type yeast expressing hemagglutinin-tagged small subunit ofTFIIE, Such a coprecipitation of the RNA polymerase subunit was seen but in a greatly reduced amount, if extract was prepared from gal11 null yeast. In light of these findings, we suggest that Gal11p stimulates promoter activity by enhancing an association of TFIIE with the preinitiation complex in the cell.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 15:22:32