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Titolo:
ACCUMULATION IN FETAL MUSCLE AND LOCALIZATION TO THE NEUROMUSCULAR-JUNCTION OF CAMP-DEPENDENT PROTEIN-KINASE-A REGULATORY AND CATALYTIC SUBUNITS RI-ALPHA AND C-ALPHA
Autore:
IMAIZUMISCHERRER T; FAUST DM; BENICHOU JC; HELLIO R; WEISS MC;
Indirizzi:
INST PASTEUR,DEPT MOL BIOL,25 RUE DR ROUX F-75724 PARIS 15 FRANCE INST PASTEUR,DEPT MOL BIOL,CNRS,UNITE GENET DIFFERENCIAT 1149 F-75724PARIS FRANCE INST PASTEUR,STN MICROSCOPIE CONFOCALE F-75724 PARIS FRANCE
Titolo Testata:
The Journal of cell biology
fascicolo: 5, volume: 134, anno: 1996,
pagine: 1241 - 1254
SICI:
0021-9525(1996)134:5<1241:AIFMAL>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
NICOTINIC ACETYLCHOLINE-RECEPTOR; PREFERENTIAL SYNAPTIC EXPRESSION; TISSUE-SPECIFIC EXTINGUISHER; IN-SITU HYBRIDIZATION; GENE-RELATED PEPTIDE; MESSENGER-RNA LEVELS; EPSILON-SUBUNIT; GAMMA-SUBUNIT; SIGNAL-TRANSDUCTION; SKELETAL-MUSCLE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
67
Recensione:
Indirizzi per estratti:
Citazione:
T. Imaizumischerrer et al., "ACCUMULATION IN FETAL MUSCLE AND LOCALIZATION TO THE NEUROMUSCULAR-JUNCTION OF CAMP-DEPENDENT PROTEIN-KINASE-A REGULATORY AND CATALYTIC SUBUNITS RI-ALPHA AND C-ALPHA", The Journal of cell biology, 134(5), 1996, pp. 1241-1254

Abstract

Using probes specific for cAMP-dependent protein kinase, we have analyzed by in situ hybridization the patterns of expression of regulatoryand catalytic subunits in mouse embryos and in adult muscle. RI alphatranscripts are distributed in muscle fibers exactly as acetylcholinesterase, showing that this RNA is localized at the neuromuscular junction. The transcript levels increase upon denervation of the muscle, but the RNA remains localized, indicating a regulation pattern similar to that of the epsilon subunit of nicotinic acetylcholine receptor. RI alpha transcripts have accumulated in the muscle by day 12 of mouse embryogenesis, and localization is established by day 14, at about the time of formation of junctions. This localization is maintained throughout development and in the adult. Immunocytochemical analysis has demonstrated that RI alpha protein is also localized. In addition, RI alpha recruits C alpha protein to the junction, providing at this site thepotential for local responsiveness to cAMP, PKA could be implicated in the establishment and/or maintenance of the unique pattern of gene expression occurring at the junction, or in the modulation of synaptic activity via protein phosphorylation. Embryonic skeletal muscle shows a high level of C alpha transcripts and protein throughout the fiber; the transcripts are already present by day 12 of embryogenesis, and their elevated level is maintained only through fetal life, In the adult, the C alpha hybridization signal of muscle is weak and homogeneous.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 14:09:58