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Titolo:
EXPRESSION OF ALPHA-SUBUNIT GENES OF NICOTINIC ACETYLCHOLINE-RECEPTORS IN HUMAN-LYMPHOCYTES
Autore:
HIEMKE C; STOLP M; REUSS S; WEVERS A; REINHARDT S; MAELICKE A; SCHLEGEL S; SCHRODER H;
Indirizzi:
UNIV MAINZ,DEPT PSYCHIAT,UNTERE ZAHLBACHER STR 8 D-55101 MAINZ GERMANY UNIV MAINZ,DEPT ANAT D-55101 MAINZ GERMANY UNIV MAINZ,DEPT PHYSIOL CHEM D-55101 MAINZ GERMANY UNIV COLOGNE,DEPT ANAT D-5000 COLOGNE GERMANY
Titolo Testata:
Neuroscience letters
fascicolo: 2-3, volume: 214, anno: 1996,
pagine: 171 - 174
SICI:
0304-3940(1996)214:2-3<171:EOAGON>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
PARKINSONS-DISEASE; BINDING; CHOLINOCEPTORS; ALZHEIMERS; SYSTEM; RAT;
Keywords:
ACETYLCHOLINE RECEPTOR; LYMPHOCYTES; NICOTINIC ALPHA-SUBUNITS; WF6 ANTIBODY; IMMUNOSTAINING; IN SITU HYBRIDIZATION; NEUROIMMUNOLOGY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
C. Hiemke et al., "EXPRESSION OF ALPHA-SUBUNIT GENES OF NICOTINIC ACETYLCHOLINE-RECEPTORS IN HUMAN-LYMPHOCYTES", Neuroscience letters, 214(2-3), 1996, pp. 171-174

Abstract

Using immunohistochemistry and in situ hybridisation, we have studiedwhether alpha-subunits of nicotinic acetylcholine receptors (nAChRs) are expressed in human lymphocytes. Cells were isolated by differential low speed gradient centrifugation from heparinised venous blood of 10 healthy volunteers. Receptor sites were visualised using the monoclonal antibody WF6 which specifically recognises alpha-isoforms from several species including man. For visualisation of transcripts, digoxigenin-labelled cRNA probes for alpha 4- and alpha 3-subunits were used. Immunostaining revealed specific binding of WF6 to isolated human lymphoid cells. The antibody was bound to most cells and concentrated preferentially in the perinuclear/surface region. The immunoreactivity resembled that observed after application of an antibody recognising CD4 surface proteins which was conducted for comparison. In situ-hybridisation revealed that the alpha 4-subunit genes of nAChRs was expressed in lymphocytes of all probands. The alpha 3-subunit was found, with lower intensity than alpha 4-transcripts, in eight of the 10 individuals. Control incubations with corresponding sense cRNAs were negative. It is concluded that human lymphocytes are able to express alpha-subunit genes of nAChRs.

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Documento generato il 26/01/20 alle ore 01:23:59