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Titolo:
UNESTERIFIED CHOLESTEROL CONTENT OF HUMAN SPERM REGULATES THE RESPONSE OF THE ACROSOME TO THE AGONIST, PROGESTERONE
Autore:
ZARINTASH RJ; CROSS NL;
Indirizzi:
OKLAHOMA STATE UNIV,DEPT PHYSIOL SCI STILLWATER OK 74078 OKLAHOMA STATE UNIV,DEPT PHYSIOL SCI STILLWATER OK 74078
Titolo Testata:
Biology of reproduction
fascicolo: 1, volume: 55, anno: 1996,
pagine: 19 - 24
SICI:
0006-3363(1996)55:1<19:UCCOHS>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIGAND-RECEPTOR EXPRESSION; ALBUMIN-MEDIATED CHANGES; HUMAN FOLLICULAR-FLUID; PLASMA-MEMBRANE; HUMAN-SPERMATOZOA; INVITRO CAPACITATION; FERTILIZING ABILITY; LIPID TRANSFER; BOVINE SPERM; GUINEA-PIG;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
R.J. Zarintash e N.L. Cross, "UNESTERIFIED CHOLESTEROL CONTENT OF HUMAN SPERM REGULATES THE RESPONSE OF THE ACROSOME TO THE AGONIST, PROGESTERONE", Biology of reproduction, 55(1), 1996, pp. 19-24

Abstract

Human sperm become responsive to inducers of the acrosome reaction ifthey are washed free of seminal plasma and incubated in an appropriate medium. We tested the hypothesis that sperm must lose cholesterol during incubation in order to become responsive to the agonist, progesterone. Freshly ejaculated sperm contained 2.92 +/- 0.202 nmol unesterified cholesterol/10(7) sperm (mean +/- SEM, n = 18), When incubated for24 h in vitro, sperm suspensions lost 29 +/- 6% of their free cholesterol (n = 23). Sperm lost cholesterol slightly faster than they becameacrosomally responsive. Adding cholesterol to the medium prevented sperm from losing cholesterol and from becoming responsive. Varying the cholesterol content of the medium had similar effects on loss of spermcholesterol (ED(50) = 406 nM) and acrosomal responsiveness (ED(50) = 388 nM), Incubating sperm with a 1:150 dilution of seminal plasma (containing 5.18 mu M cholesterol) also prevented sperm from losing cholesterol and from becoming responsive. Incubating sperm 24 h in medium containing 0.5 mg/ml phosphatidylcholine increased the amount of cholesterol lost and the number of sperm that became responsive, Our results support a model in which sperm unesterified cholesterol (or a moleculein equilibrium with it) suppresses acrosomal responsiveness. Sperm must lose unesterified cholesterol to become responsive to progesterone.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 03:17:06