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Titolo:
FRITLESS PACKED-COLUMNS FOR CAPILLARY ELECTROCHROMATOGRAPHY - SEPARATION OF UNCHARGED COMPOUNDS ON HYDROPHOBIC HYDROGELS
Autore:
FUJIMOTO C; FUJISE Y; MATSUZAWA E;
Indirizzi:
HAMAMATSU UNIV,SCH MED,DEPT CHEM HAMAMATSU SHIZUOKA 43131 JAPAN HAMAMATSU PHOTON KK HAMAMATSU SHIZUOKA 43131 JAPAN
Titolo Testata:
Analytical chemistry
fascicolo: 17, volume: 68, anno: 1996,
pagine: 2753 - 2757
SICI:
0003-2700(1996)68:17<2753:FPFCE->2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; REVERSED-PHASE CHROMATOGRAPHY; POLYACRYLAMIDE GELS; ELECTROOSMOTIC FLOW; CONTINUOUS BEDS; ENANTIOMER SEPARATION; ELECTROPHORESIS; CYCLODEXTRIN; PROFILES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
C. Fujimoto et al., "FRITLESS PACKED-COLUMNS FOR CAPILLARY ELECTROCHROMATOGRAPHY - SEPARATION OF UNCHARGED COMPOUNDS ON HYDROPHOBIC HYDROGELS", Analytical chemistry, 68(17), 1996, pp. 2753-2757

Abstract

A novel column is described that does not require frits to keep packing material within a capillary, A continuous bed is prepared in situ in aqueous solution by radical copolymerization of N-isopropylacrylamide and 2-acrylamido-2-methylpropanesulfonic acid (the resultant gel is denoted poly(AMPS-co-IPAAm). N,N'-Methylenebisacrylamide is used for cross-linking, On the application of an electrical field, electroosmotic now (EOF) is developed in the bed along the capillary, where fluid propulsion would be otherwise difficult to achieve, The resultant EOF transports neutral compounds through the column without forcing the gelout of the capillary, Examination of the fluid motion in the continuous bed using a video microscope system and an image processor shows a relatively nat now profile of EOF. The bed functions as the stationaryphase for reversed-phase capillary electrochromatography (CEC), This new approach is an alternative to packed capillary columns which have been used previously in CEC, A high efficiency is obtained for a steroid which is separated on a 4.0% total monomer concentration (T), 10.0%degree of cross-linking (C), and 10.0% mole fraction of AMPS in the total monomer (S), poly(AMPS-co-IPAAm) column. A mixture of polyaromatic hydrocarbons is separated on a 6.9% T, 5.8% C, and 5.5% S poly(AMPS-co-IPAAm) column. The capacity factor of benzo[a]pyrene increases from0.63 to 1.91 as the acetonitrile content in a Tris-boric acid buffer is decreased from 45 to 30% (v/v), The run-to-run RSD of analyte migration time is less than 0.73%, and the day-to-day RSD is acceptable, Potential benefits of this approach are also mentioned.

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Documento generato il 02/12/20 alle ore 17:42:15