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Titolo:
CHARACTERIZATION OF AN IL-6-LIKE MOLECULE FROM AN ECHINODERM (ASTERIAS-FORBESI)
Autore:
BECK G; HABICHT GS;
Indirizzi:
UNIV MASSACHUSETTS,DEPT BIOL BOSTON MA 02125 SUNY STONY BROOK,DEPT PATHOL STONY BROOK NY 11794
Titolo Testata:
Cytokine
fascicolo: 7, volume: 8, anno: 1996,
pagine: 507 - 512
SICI:
1043-4666(1996)8:7<507:COAIMF>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; INVERTEBRATE CYTOKINES; INTERLEUKIN-1; PURIFICATION; PROTEIN; IMMUNE; CELLS;
Keywords:
CYTOKINE; EVOLUTION; IMMUNOLOGY; HOST DEFENSE; INVERTEBRATE; IL-6; CELOMOCYTES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
G. Beck e G.S. Habicht, "CHARACTERIZATION OF AN IL-6-LIKE MOLECULE FROM AN ECHINODERM (ASTERIAS-FORBESI)", Cytokine, 8(7), 1996, pp. 507-512

Abstract

Our previous studies have demonstrated the presence of two of the three vertebrate inflammatory cytokines in invertebrates, namely, interleukin (IL)-1 and tumour necrosis factor (TNF), We studied the coelomic fluid of the echinoderm, Asterias forbesi, to determine whether it contained the third inflammatory cytokine, IL-6, Coelomic fluids were concentrated and then fractionated by a combination of gel sieve chromatography and preparative isoelectric focusing, The M(r) observed for theinvertebrate IL-6-like molecule was approximate to 30 000 and the pI was 5.5, Western blot analysis using a polyclonal rabbit antiserum to human IL-6 detected a band at approximate to 30 000 Da, The invertebrate IL-6 was inhibited by an antiserum to human IL-6 when used in the B9 assay Finally, the coelomocytes were found to be capable of releasing the IL-6-like molecule as early as 12 h following stimulation by LPS, These results, together with our other data, show that echinoderms possess correlates of all three vertebrate inflammatory cytokines. (C) 1996 Academic Press Limited

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/05/20 alle ore 15:09:58