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Titolo:
RAPID-DETERMINATION OF IN-VIVO AND IN-VITRO ANTIBODY-RESPONSES BY SUSPENSION HEMOLYTIC ASSAY
Autore:
HAN SB; OH GT; YUN YP; MIN BK; HYUN BH; KIM HM;
Indirizzi:
KIST,KOREA RES INST BIOSCI & BIOTECHNOL,POB 115 TAEJON 305600 SOUTH KOREA KIST,KOREA RES INST BIOSCI & BIOTECHNOL TAEJON 305600 SOUTH KOREA CHUNGBUK NATL UNIV,DEPT PHARM CHONJU 360763 SOUTH KOREA
Titolo Testata:
Journal of pharmacological and toxicological methods
fascicolo: 1, volume: 36, anno: 1996,
pagine: 33 - 40
SICI:
1056-8719(1996)36:1<33:ROIAIA>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUPPRESSION; LYMPHOCYTE; CELLS;
Keywords:
SH ASSAY; PFC ASSAY; FCS; DIOXIN; GLYCOPEPTIDE; ANTIBODY RESPONSES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
S.B. Han et al., "RAPID-DETERMINATION OF IN-VIVO AND IN-VITRO ANTIBODY-RESPONSES BY SUSPENSION HEMOLYTIC ASSAY", Journal of pharmacological and toxicological methods, 36(1), 1996, pp. 33-40

Abstract

A suspension hemolytic (SH) assay newly modified in this laboratory was applied to the measurement of primary IgM antibody responses, such as T-dependent, T-independent, and polyclonal B cell. responses in in vivo and in vitro assays. All results of the SH assay were equal to those of the conventional plaque-forming cell (PFC) assay. With the SH assay in in vitro assays, we could separately measure the amount of theIgM that had been produced during the whole immunization period and measure the IgM newly produced during the shorter time of the assay. The immunomodulations by dioxin and glycopeptide were accurately evaluated by the SH and the PFC assay. Our study suggested that the SH assay modified in this laboratory was more advantageous because of the simplicity of the assay and better analysis of data than the PFC assay, andcould be used as the primary method in studying the effects of pharmaceutical and toxic agents on humoral immune functions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 14:31:32