Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
PLASMID-AMPLIFIED COMS ENHANCES GENETIC COMPETENCE AND SUPPRESSES SINR IN BACILLUS-SUBTILIS
Autore:
LIU L; NAKANO MM; LEE OH; ZUBER P;
Indirizzi:
LOUISIANA STATE UNIV,CTR MED,DEPT BIOCHEM & MOL BIOL,1501 KINGS HIGHWAY SHREVEPORT LA 71130 LOUISIANA STATE UNIV,CTR MED,DEPT BIOCHEM & MOL BIOL SHREVEPORT LA 71130
Titolo Testata:
Journal of bacteriology
fascicolo: 17, volume: 178, anno: 1996,
pagine: 5144 - 5152
SICI:
0021-9193(1996)178:17<5144:PCEGCA>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA-BINDING PROTEIN; SIGNAL-TRANSDUCTION; SURFACTIN SYNTHETASE; TRANSCRIPTION FACTOR; SRFA OPERON; NUCLEOTIDE-SEQUENCE; MUTATIONAL ANALYSIS; ACTIVATING DOMAIN; REGULATOR; SPORULATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
65
Recensione:
Indirizzi per estratti:
Citazione:
L. Liu et al., "PLASMID-AMPLIFIED COMS ENHANCES GENETIC COMPETENCE AND SUPPRESSES SINR IN BACILLUS-SUBTILIS", Journal of bacteriology, 178(17), 1996, pp. 5144-5152

Abstract

The establishment of genetic competence in Bacillus subtilis is controlled by a vast signal transduction network involving the products of genes that function in several postexponential-phase processes. Two ofthese proteins, SinR and DegU, serve as molecular switches that influence a cell's decision to undergo either sporulation or genetic competence development. In order to determine the roles of SinR and DegU in competence control, multicopy suppression experiments with plasmid-amplified comS, sinR, and degU genes were undertaken. Multicopy comS was found to elevate competence gene transcription and transformation efficiency in both wild-type and sinR mutant cells but not in degU mutant cells. Multicopy degU failed to suppress comS or sinR mutations. No suppression of comS or degU by multicopy sinR was observed. The expression of a comS'::'lacZ translational fusion and srf-lacZ operon fusion was examined in sinR cells and cells bearing plasmid-amplified sinR. The expression of comS'::'lacZ gene fusion was reduced hy the sinR mutation, but both comS'::'lacZ and srf-lacZ were repressed by multicopy sinR. Cells hearing plasmid-amplified sinR were poorly competent. These results suggest that sinR is required for optimal comS expression but not transcription from the srf promoter and that SinR at high concentrations represses srf transcription initiation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 04:28:55