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Titolo:
ANALYSIS OF IL-2 RECEPTOR EXPRESSION AND OF THE BIOLOGICAL EFFECTS OFIL-2 GENE TRANSFECTION IN SMALL-CELL LUNG-CANCER
Autore:
MEAZZA R; MARCIANO S; SFORZINI S; ORENGO AM; COPPOLECCHIA M; MUSIANI P; ARDIZZONI A; SANTI L; AZZARONE B; FERRINI S;
Indirizzi:
IST NAZL RIC CANC,LARGO ROSANNA BENZI 10 I-16132 GENOA ITALY IST NAZL RIC CANC I-16132 GENOA ITALY UNIV GENOA,IST ONCOL CLIN & SPERIMENTALE I-16132 GENOA ITALY UNIV CHIETI,IST PATOL UMANA & MED SOCIALE I-66100 CHIETI ITALY HOP PAUL BROUSSE,UNITE INSERM 268 F-94800 VILLEJUIF FRANCE
Titolo Testata:
British Journal of Cancer
fascicolo: 5, volume: 74, anno: 1996,
pagine: 788 - 795
SICI:
0007-0920(1996)74:5<788:AOIREA>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; HUMAN-MELANOMA CELLS; MURINE MAMMARY ADENOCARCINOMA; NATURAL-KILLER-CELLS; INTERLEUKIN-2 RECEPTOR; FACTOR-ALPHA; ANTITUMOR IMMUNITY; LYMPHOCYTES-T; TUMORIGENICITY; MACROPHAGES;
Keywords:
INTERLEUKIN-2 RECEPTOR; TRANSFECTION; LUNG CANCER; INTERLEUKIN 2; XENOTRANSPLANT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
R. Meazza et al., "ANALYSIS OF IL-2 RECEPTOR EXPRESSION AND OF THE BIOLOGICAL EFFECTS OFIL-2 GENE TRANSFECTION IN SMALL-CELL LUNG-CANCER", British Journal of Cancer, 74(5), 1996, pp. 788-795

Abstract

We have analysed the expression of interleukin-2 receptor (IL-2R) on a panel of small-cell lung cancer (SCLC) cell lines. None of the 11 SCLC cell lines studied expressed detectable surface IL-2R alpha or betachains by indirect immunofluorescence. Reverse transcriptase-polymerase chain reaction (RT-PCR) analyses indicated that only one out of 11 cell lines expressed detectable IL-2R beta mRNA while two expressed a weak positivity for IL-2R gamma. Five SCLC cell lines were transfectedwith the plasmid vector RSV.5 neo containing IL-2 cDNA coding. sequence. Stable transfectants secreted biologically active IL-2 (ranging From 25 to 100 U ml(-1) in the culture supernatant). IL-2, transfection did not product significant modifications in the expression of surfacemolecules such as IL-2R alpha and beta chains, intercellular adhesionmolecule-1 (ICAM-1), CD44, HLA class I and II or in IL-2R beta or gamma mRNA. More importantly, IL-2-transfected N592 and NCI H69 cell lines completely lost their tumorigenic potential in nude mice after subcutaneous injection, whereas experimental controls transfected with RSV.5 neo vector only, displayed an in vivo growth pattern identical to that of untransfected cells. In addition, in the N592 model. IL-2-producing N592 inhibited the growth of wild-type N592 injected at the same site, while injection of parental cells on the opposite side did not significantly affect the growth of wild-type tumour cells. Histopathological analysis of the rejection process of IL-2-transfected cells demonstrated the presence of MAC-1(+), MAC-3(+) macrophages and of RB68C5(+) granulocytes, whereas T cells were undetectable and NK cells were scarcely represented. In addition, a reduction of the tumour blood vessels was observed. The possible relevance of these data for the development of vaccination strategies using cytokine-engineered tumour cells in SCLC is discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 19:47:47