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Titolo:
CONSERVED NUCLEOTIDES OF 23 S RIBOSOMAL-RNA LOCATED AT THE RIBOSOMAL PEPTIDYLTRANSFERASE CENTER
Autore:
SPAHN CMT; SCHAFER MA; KRAYEVSKY AA; NIERHAUS KH;
Indirizzi:
AG RIBOSOMEN,MAX PLANCK INST MOL GENET,IHNESTR 73 D-14195 BERLIN GERMANY AG RIBOSOMEN,MAX PLANCK INST MOL GENET D-14195 BERLIN GERMANY RUSSIAN ACAD SCI,VA ENGELHARDT MOL BIOL INST MOSCOW 117984 RUSSIA
Titolo Testata:
The Journal of biological chemistry
fascicolo: 51, volume: 271, anno: 1996,
pagine: 32857 - 32862
SICI:
0021-9258(1996)271:51<32857:CNO2SR>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
PEPTIDYL TRANSFERASE CENTER; ALLOSTERIC 3-SITE MODEL; TRANSFER-RNA; ELONGATION CYCLE; MESSENGER-RNA; P-SITES; THIOSTREPTON; MUTAGENESIS; VIOMYCIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
C.M.T. Spahn et al., "CONSERVED NUCLEOTIDES OF 23 S RIBOSOMAL-RNA LOCATED AT THE RIBOSOMAL PEPTIDYLTRANSFERASE CENTER", The Journal of biological chemistry, 271(51), 1996, pp. 32857-32862

Abstract

Two nucleotides of the 23 S rRNA gene were mutated; the nucleotides correspond to the first two positions of the universally conserved sequence Psi GG2582 at the peptidyltransferase ring of 23 S rRNA. The ribosomes containing the altered 23 S rRNA were analyzed. Previously, it was shown that ribosomal assembly was indistinguishable from that in wild-type cells, that the flow of the corresponding 50 S subunit into the polysome fraction was not restricted, but that the ribosomes were strongly impaired in poly(Phe) synthesis (C. M. T. Spahn, J. Remme, M. A. Schafer, and K. H. Nierhaus (1996) J. Biol. Chem. 271, 32849-32856). Here we apply assay systems exclusively testing the puromycin reaction of ribosomes carrying plasmid-born rRNA, a dipeptide assay using theminimal P site donor pA(fMet) and a translocation system not depending on the puromycin reaction. The mutations in helix 90 exclusively abolish or severely impair the ribosome capability to catalyze AcPhe-puromycin formation, A possible explanation of these observations is that G2581 and Psi 2580 (and possibly also G2582) are part of the binding site of C75 of peptidyl-tRNA in the P site. The results suggest that inthis case, however, such an interaction would disobey canonical base pairing.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 01:35:22