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Titolo:
MUTATIONAL ANALYSIS OF 2 HIGHLY CONSERVED UGG SEQUENCES OF 23 S RIBOSOMAL-RNA FROM ESCHERICHIA-COLI
Autore:
SPAHN CMT; REMME J; SCHAFER MA; NIERHAUS KH;
Indirizzi:
AG RIBOSOMEN,MAX PLANCK INST MOL GENET,IHNESTR 73 D-14195 BERLIN GERMANY AG RIBOSOMEN,MAX PLANCK INST MOL GENET D-14195 BERLIN GERMANY TARTU STATE UNIV,INST MOL & CELL BIOL EE-2400 TARTU ESTONIA
Titolo Testata:
The Journal of biological chemistry
fascicolo: 51, volume: 271, anno: 1996,
pagine: 32849 - 32856
SICI:
0021-9258(1996)271:51<32849:MAO2HC>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
PEPTIDYL TRANSFERASE CENTER; FUNCTIONAL TRANSFER-RNAS; RIBOSOMAL-RNA; PEPTIDYLTRANSFERASE ACTIVITY; A-SITES; P-SITES; MUTAGENESIS; SUBUNIT; INVIVO; RECONSTITUTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
52
Recensione:
Indirizzi per estratti:
Citazione:
C.M.T. Spahn et al., "MUTATIONAL ANALYSIS OF 2 HIGHLY CONSERVED UGG SEQUENCES OF 23 S RIBOSOMAL-RNA FROM ESCHERICHIA-COLI", The Journal of biological chemistry, 271(51), 1996, pp. 32849-32856

Abstract

The 23 S-type rRNA contains two phylogenetically conserved UGG sequences, which have the potential to bind the universal CCA-3'-ends of tRNAs at the ribosomal peptidyltransferase center by base pairing. The first two positions, UG, of these sequences at the helix-loop 80 (U2249G2250) and helix-loop 90 (Psi 2580G2581) and some related nucleotides were tested by site-directed mutagenesis for their involvement in ribosomal function, i.e. peptidyltransferase. The plasmid derived mutated 23 S rRNA comprised about 50% of the total 23 S rRNA, None of the single mutations caused an assembly defect, and all 50 S subunits carrying an altered 23 S rRNA could freely exchange with the pools of 70S ribosomes and polysomes, The mutations at the helix-loop 80 region hardly affected bacterial growth. However, mutations at the helix 90 caused severe growth effects and severely impaired the in vitro protein synthesis, showing that this 23 S rRNA region is of high importance for ribosomal function.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 07:06:17