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Titolo:
LINEAR FUSION OF HIV-1 B-EPITOPES AND HETEROLOGOUS T-CELL EPITOPES .2. MULTIPARAMETRIC ASSAY OF IMMUNOGENICITY OF COMPOSITE PEPTIDES IN RABBITS
Autore:
ISAGULIANTS MG; RUDEN U; WAHREN B;
Indirizzi:
RUSSIAN ACAD MED SCI,DI IVANOVSKII VIROL INST,UL GAMALEI 16 MOSCOW 123098 RUSSIA SWEDISH INST INFECT DIS CONTROL,DEPT VIROL S-10521 STOCKHOLM SWEDEN KAROLINSKA INST,DEPT MICROBIOL & TUMOR BIOL S-10521 STOCKHOLM SWEDEN
Titolo Testata:
Biochemistry
fascicolo: 7, volume: 61, anno: 1996,
pagine: 878 - 885
SICI:
0006-2979(1996)61:7<878:LFOHBA>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
GP41; IDENTIFICATION; SEQUENCES;
Keywords:
B-EPITOPE T-CELL EPITOPE; HIV-1; COMPOSITE PEPTIDES; IMMUNOGENICITY; ANTIBODY PRODUCTION; T-CELL PROLIFERATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
17
Recensione:
Indirizzi per estratti:
Citazione:
M.G. Isaguliants et al., "LINEAR FUSION OF HIV-1 B-EPITOPES AND HETEROLOGOUS T-CELL EPITOPES .2. MULTIPARAMETRIC ASSAY OF IMMUNOGENICITY OF COMPOSITE PEPTIDES IN RABBITS", Biochemistry, 61(7), 1996, pp. 878-885

Abstract

Rabbits were immunized with mixtures of linearly connected peptides (composites) combining an immunodominant B-cell epitope from gp41 of HIV-1 (amino acids 593-604) and a heterogenous T-cell epitope from tetanus toroid or hepatitis B core antigen. The HIV-1 B-epitope in the context of the composites induced HIV-1-specific antibody response, while no antibodies were produced in response to the peptide representing the single HIV-1 B-epitope. Competition ELISA and ELISA of HIV-1 peptideomission analogs demonstrated that immunization induced antibodies ofthe correct specificity. T-cell epitope components also elicited potent antibody production. The T-cell epitopes appeared to be immunodominant. However, HIV-1-specific antibody response was not suppressed; on the contrary, antibody response against the HIV-1 B-epitope and the heterologous T-cell epitopes developed in parallel. Cellular responses against composite peptides and/or their components were induced in all animals. The ability to develop cellular response against T-cell epitopes from the composites determined the efficacy of humoral response against the composites, including the HIV-1-specific one. Animals with strong cellular responses rapidly developed maximal antipeptide antibody titers. The immunization results imply that linear fusion of B- and T-cell epitopes yielded bivalent, multi-epitope products in which the immunological properties of the individual components were maintained and desired immunogenic properties were obtained. The immunogenicity of the composite peptides corresponded to that expected from the study of the composite antigenic properties.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 14:28:16