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Titolo:
PROGRESSIVE DECREASE IN V(H)3 GENE FAMILY EXPRESSION IN PLASMA-CELLS OF HIV-INFECTED PATIENTS
Autore:
DAVID D; DEMAISON C; BANI L; THEZE J;
Indirizzi:
INST PASTEUR,DEPT IMMUNOL,UNITE IMMUNOGENET CELLULAIRE,28 RUE DR ROUXF-75724 PARIS 15 FRANCE INST PASTEUR,DEPT IMMUNOL,UNITE IMMUNOGENET CELLULAIRE F-75724 PARIS 15 FRANCE
Titolo Testata:
International immunology
fascicolo: 8, volume: 8, anno: 1996,
pagine: 1329 - 1333
SICI:
0953-8178(1996)8:8<1329:PDIVGF>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN MONOCLONAL-ANTIBODIES; B-CELLS; PROTEIN-A; IN-VIVO; LYMPHOCYTES; REPERTOIRE; POLYSACCHARIDE; IGM;
Keywords:
B CELL SUPERANTIGEN; HIV INFECTION; PLASMA CELL REPERTOIRE; V(H)3 GENE FAMILY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
D. David et al., "PROGRESSIVE DECREASE IN V(H)3 GENE FAMILY EXPRESSION IN PLASMA-CELLS OF HIV-INFECTED PATIENTS", International immunology, 8(8), 1996, pp. 1329-1333

Abstract

We have analysed the expression of V-H gene families in total peripheral plasma cells of mu and gamma isotypes from a group of HIV-infectedpatients. CD19(-) and CD20(-) cells were separated from B lymphocytes, and anchored RT-PCR products were hybridized with specific V-H gene family probes and with a consensus J(H) region probe. The V-H/J(H) hybridization intensity ratios were taken as a parameter to measure V-H expression. In vivo V(H)3 gene family expression is reduced in plasma cells of all HIV-infected patients compared with adult healthy donors, This decrease is maximal in patients with AIDS: >90%. This is true forboth studied isotypes. Conversely, the two other main V-H gene families, V(H)1 and V(H)4, show no significant variation in expression. We and others have previously shown that V(H)3 gene family expression is first expanded and then decreased in peripheral B lymphocytes of HIV-infected patients, The present results extend these observations and show that V(H)3 gene family expression is also affected in plasma cells. The existence of a B cell superantigen in HIV infection may explain these data, This pronounced reduction in V(H)3 family expression may participate in the impaired humoral responses against bacterial agents found in HIV-infected patients.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 11:51:00