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Titolo:
DIFFERENTIAL TARGETING OF FACILITATIVE GLUCOSE TRANSPORTERS IN POLARIZED EPITHELIAL-CELLS
Autore:
PASCOE WS; INUKAI K; OKA Y; SLOT JW; JAMES DE;
Indirizzi:
UNIV QUEENSLAND,CTR CELLULAR & MOL BIOL ST LUCIA QLD 4072 AUSTRALIA UNIV QUEENSLAND,CTR CELLULAR & MOL BIOL ST LUCIA QLD 4072 AUSTRALIA ASHAHI LIFE FDN,INST ADULT DIS TOKYO JAPAN YAMAGUCHI UNIV,SCH MED,DEPT INTERNAL MED 3 YAMAGUCHI JAPAN UNIV UTRECHT,SCH MED,DEPT CELL BIOL NL-3584 CX UTRECHT NETHERLANDS
Titolo Testata:
American journal of physiology. Cell physiology
fascicolo: 2, volume: 40, anno: 1996,
pagine: 547 - 554
SICI:
0363-6143(1996)40:2<547:DTOFGT>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMA-MEMBRANE; INTRACELLULAR-TRANSPORT; CELLULAR-LOCALIZATION; CYTOPLASMIC DOMAIN; MDCK CELLS; EXPRESSION; GLUT3; PROTEINS; CLONING; TRANSLOCATION;
Keywords:
MADIN-DARBY CANINE KIDNEY CELLS; PROTEIN TARGETING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
W.S. Pascoe et al., "DIFFERENTIAL TARGETING OF FACILITATIVE GLUCOSE TRANSPORTERS IN POLARIZED EPITHELIAL-CELLS", American journal of physiology. Cell physiology, 40(2), 1996, pp. 547-554

Abstract

We have examined the intracellular localization of five facilitative glucose transporter proteins, one endogenous (GLUT-1) and four exogenous (GLUT-2, -3, -4, and -5), in polarized epithelial cells. GLUT-2, -3, -4, and -5 were stably transfected into Madin-Darby canine kidney (MDCK) cells, and peptide-specific antibodies were used to establish their distribution by immunofluorescence and immunoelectron-microscopic techniques. GLUT-1 and -2 were predominantly targeted to the basolateral domain of the cell, whereas GLUT-3 and -5 were targeted to the apical plasma membrane. The insulin-regulatable glucose transporter GLUT-4 was found in intracellular tubulovesicular structures beneath the surface of the cell. Vectorial 2-deoxy-D-glucose uptake measurements revealed that similar to 95% of glucose entry into wild-type MDCK cells occurs via the basolateral membrane. In GLUT-3-transfected cells, however, apical glucose uptake increased to similar to 55%; this was not observed in cells expressing the other GLUT isoforms. The discrete and differential intracellular localizations of the various GLUTs, in addition to the high level of sequence homology and predicted secondary structure similarity, ren der the GLUT family ideal for the study of intrinsic targeting motifs involved in the establishment and maintenance of cellular polarity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 19:50:12