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Titolo:
ACTIVATION OF KERATINOCYTE NICOTINIC CHOLINERGIC RECEPTORS STIMULATESCALCIUM INFLUX AND ENHANCES CELL-DIFFERENTIATION
Autore:
GRANDO SA; HORTON RM; MAURO TM; KIST DA; LEE TX; DAHL MV;
Indirizzi:
UNIV CALIF DAVIS,MED CTR,DEPT DERMATOL,1605 ALHAMBRA BLVD SACRAMENTO CA 95816 UNIV MINNESOTA,DEPT DERMATOL MINNEAPOLIS MN 55455 UNIV CALIF SAN FRANCISCO,DEPT DERMATOL SAN FRANCISCO CA 94143
Titolo Testata:
Journal of investigative dermatology
fascicolo: 3, volume: 107, anno: 1996,
pagine: 412 - 418
SICI:
0022-202X(1996)107:3<412:AOKNCR>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACETYLCHOLINE-RECEPTOR; TERMINAL DIFFERENTIATION; EPIDERMAL-CELLS; CHANNEL; PERMEABILITY; PRECURSOR; FILAGGRIN; CULTURE; BLOCKS; SITES;
Keywords:
ACETYLCHOLINE RECEPTORS; KERATINOCYTE DIFFERENTIATION MARKERS; IMMUNOCYTOCHEMISTRY; IMMUNOBLOTTING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
S.A. Grando et al., "ACTIVATION OF KERATINOCYTE NICOTINIC CHOLINERGIC RECEPTORS STIMULATESCALCIUM INFLUX AND ENHANCES CELL-DIFFERENTIATION", Journal of investigative dermatology, 107(3), 1996, pp. 412-418

Abstract

Human epidermal keratinocytes synthesize, secrete, and degrade acetylcholine and use their cell-surface nicotinic and muscarinic cholinergic receptors to mediate the autocrine and paracrine effects of acetylcholine, Because acetylcholine modulates transmembrane Ca2+ transport and intracellular metabolism in several types of cells, we hypothesized that cholinergic agents might have similar effects on keratinocytes, Nicotine increased in a concentration-dependent manner the amount of Ca-45(2+) taken up by keratinocytes isolated from human neonatal foreskins, This effect was abolished in the presence of the specific nicotinic antagonist mecamylamine, indicating that it was mediated by keratinocyte nicotinic acetylcholine receptor(s), The sequences encoding the alpha 5 and alpha 7 nicotinic receptor subunits were amplified from cDNA isolated from cultured keratinocytes, These subunits, as well as thealpha 3, beta 2, and beta 4 subunits previously found in keratinocytes, can be components of Ca2+-permeable nicotinic receptor channels. Tolearn how activation of keratinocyte nicotinic receptors affected therate of cell differentiation, we measured the nicotinic cholinergic effects on the expression of differentiation markers by cultured keratinocytes. Long-term incubations with micromolar concentrations of nicotine markedly increased the number of cells forming cornified envelopesand the number of cells staining with antibodies to suprabasal keratin 10, transglutaminase type I, involucrin, and filaggrin. The increased production of these differentiation-associated proteins was verifiedby Western blotting, Because nicotinic cholinergic stimulation causestransmembrane Ca2+ transport into keratinocytes, and because changes in concentrations of intracellular Ca2+ are known to alter various keratinocyte functions, including differentiation, the subcellular mechanisms mediating the autocrine and paracrine actions of epidermal acetylcholine on keratinocytes may involve Ca2+ as a second messenger.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 10:18:21