Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
CHARACTERIZATION OF MATRIX METALLOPROTEINASE (MMP-8 AND MMP-9) ACTIVITIES IN THE SALIVA AND IN GINGIVAL CREVICULAR FLUID OF CHILDREN WITH DOWNS-SYNDROME
Autore:
HALINEN S; SORSA T; DING YL; INGMAN T; SALO T; KONTTINEN YT; SAARI H;
Indirizzi:
UNIV HELSINKI,DEPT ANAT,INST BIOMED,POB 9,SILTAVUORENPENGER 20A FIN-00014 HELSINKI FINLAND UNIV HELSINKI,DEPT PERIODONTOL FIN-00014 HELSINKI FINLAND UNIV HELSINKI,DEPT MED CHEM FIN-00014 HELSINKI FINLAND UNIV HELSINKI,DEPT ORTHODONT & PEDODONT FIN-00014 HELSINKI FINLAND UNIV OULU,DEPT PATHOL OULU FINLAND UNIV OULU,DEPT ORAL SURG OULU FINLAND UNIV HELSINKI,CENT HOSP,DEPT MED FIN-00014 HELSINKI FINLAND
Titolo Testata:
Journal of periodontology
fascicolo: 8, volume: 67, anno: 1996,
pagine: 748 - 754
SICI:
0022-3492(1996)67:8<748:COMM(A>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERIODONTAL TISSUE DESTRUCTION; IV COLLAGENASE; IN-VIVO; NEUTROPHIL; DISEASE; PURIFICATION; GELATINASES; TRYPSIN; PLAQUE; CELLS;
Keywords:
COLLAGENASE; GELATINASE; GINGIVAL CREVICULAR FLUID CHEMISTRY; SALIVA/CHEMISTRY; PERIODONTITIS/ETIOLOGY; GINGIVITIS/ETIOLOGY; DOWNS SYNDROME COMPLICATIONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
S. Halinen et al., "CHARACTERIZATION OF MATRIX METALLOPROTEINASE (MMP-8 AND MMP-9) ACTIVITIES IN THE SALIVA AND IN GINGIVAL CREVICULAR FLUID OF CHILDREN WITH DOWNS-SYNDROME", Journal of periodontology, 67(8), 1996, pp. 748-754

Abstract

PREVIOUS STUDIES HAVE SHOWN INCREASED susceptibility to periodontal diseases in children with Down's syndrome (DS), The mechanisms involvedin the periodontal inflammatory processes in DS are not fully understood. The present study characterized the periodontal status of 9 non-institutionalized DS children 9 to 17 years old (mean 13.6 years) relative to their age-matched systemically and periodontally healthy controls, The periodontal status was assessed by visible plaque index (VPI),gingival bleeding index (GBI), and probing depth. We also assessed, by sodium dodecyl sulphate polyacrylamide gel electrophoresis/laser densitometry and by zymography, the collagenase and gelatinase activitiesin the gingival crevicular fluid (GCF) and saliva samples collected from DS patients and from the controls. Eight of the nine DS children showed a periodontium comparable to that seen in healthy controls; beginning alveolar bone loss was radiographically seen In the DS patient with deep periodontal Dockets. The endogenously active collagenase and total collagenase activities were slightly higher in GCF of DS children compared to healthy controls. Western blot demonstrated that GCF collagenase of DS patients was human neutrophil collagenase (MMP-8 or collagenase-2), which occurred in 75 kDa proMMP-8 and in DS patients, butnot in controls, also in 65 kDa active MMP-8 form and occasionally lower 40-50 kDa MMP-8 species. Zymographic analysis revealed the presence of 120 kDa (MMP-9 complexed with neutrophil gelatinase associated lipocalin or NGAL), 92 kDa (MMP-9) and 72 kDa (MMP-2) gelatinases in DS and control GCE Especially in DS GCF MMP-9 occurred in part in 82-85 kDa activated form. Salivary collagenase in DS was high when compared to controls but of the same MMP-8 type as in control saliva. Our findings suggest that in vivo activated MMP-8 in GCF derived from triggered PMNs and/or cytokine-induced periodontal fibroblasts may reflect periodontal tissue and alveolar bone destruction seen in the early stages of gingivitis/periodontitis associated with Down's syndrome.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 05:27:14