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Titolo:
MATRIX METALLOPROTEINASES AND TIMPS IN CULTURED C57BL 6J-CPK KIDNEY-TUBULES/
Autore:
RANKIN CA; SUZUKI K; ITOH Y; ZIEMER DM; GRANTHAM JJ; CALVET JP; NAGASE H;
Indirizzi:
UNIV KANSAS,MED CTR,DEPT BIOCHEM & MOL BIOL KANSAS CITY KS 66160 UNIV KANSAS,MED CTR,DEPT MED KANSAS CITY KS 66160
Titolo Testata:
Kidney international
fascicolo: 3, volume: 50, anno: 1996,
pagine: 835 - 844
SICI:
0085-2538(1996)50:3<835:MMATIC>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYACRYLAMIDE-GEL-ELECTROPHORESIS; PHENOL-CHLOROFORM EXTRACTION; SINGLE-STEP METHOD; TISSUE INHIBITOR; IV COLLAGENASE; DEGRADING METALLOPROTEINASE; POLYCYSTIC DISEASE; FIBROSARCOMA CELLS; MOLECULAR-CLONING; BUFFER SYSTEMS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
63
Recensione:
Indirizzi per estratti:
Citazione:
C.A. Rankin et al., "MATRIX METALLOPROTEINASES AND TIMPS IN CULTURED C57BL 6J-CPK KIDNEY-TUBULES/", Kidney international, 50(3), 1996, pp. 835-844

Abstract

Restructuring of basement membranes is a hallmark of the pathology ofrenal cystic disorders. Here, we present findings consistent with theview that basement membrane degradation by matrix metalloproteinases (MMPs) may contribute to abnormal basement membrane structure in polycystic kidney disease. Cells from cystic kidney tubules embedded in collagen gels appeared to migrate through the gel. This migration throughcollagen indicated that these cells could degrade the matrix. To examine this activity, wt: cultured cystic kidney tubules derived from theC57BL/6J cpk/cpk mouse, a hereditary modal of polycystic kidney disease, and assayed conditioned medium for the presence of MMPs and tissueinhibitors of metalloproteinases (TIMPs). The conditioned medium fromthe cystic tubules contained higher than normal levels of MMP-9, MMP-2, and MMP-3 as well as TIMP-1 and TIMP-2. A 101 kDa protease was present equally in cystic and control cultures and although inhibited by EDTA, it was not inhibited by TIMPs, nor activated by the mereurial compound APMA. These data suggest that cystic kidney tubules synthesize and secrete high levels of MMPs which may then participate in the restructuring of the tubular basement membrane.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 16:18:55