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Titolo:
ENHANCEMENT OF PORCINE NATURAL-KILLER-CELL ACTIVITY BY RECOMBINANT HUMAN AND MURINE IL-12
Autore:
CHO DH; LEE WJ; HALLORAN PJ; TRINCHIERI G; KIM YB;
Indirizzi:
FINCH UNIV HLTH SCI CHICAGO MED SCH,DEPT MICROBIOL & IMMUNOL,3333 GREEN BAY RD N CHICAGO IL 60064 FINCH UNIV HLTH SCI CHICAGO MED SCH,DEPT MICROBIOL & IMMUNOL N CHICAGO IL 60064
Titolo Testata:
Cellular immunology
fascicolo: 1, volume: 172, anno: 1996,
pagine: 29 - 34
SICI:
0008-8749(1996)172:1<29:EOPNAB>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
LYMPHOCYTE MATURATION FACTOR; STIMULATORY FACTOR INTERLEUKIN-12; LYTIC TRIGGER MOLECULES; T RESPONSES INVITRO; HETERODIMERIC CYTOKINE; NK CELLS; PNK-E; PROLIFERATION; CLONING; IDENTIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
D.H. Cho et al., "ENHANCEMENT OF PORCINE NATURAL-KILLER-CELL ACTIVITY BY RECOMBINANT HUMAN AND MURINE IL-12", Cellular immunology, 172(1), 1996, pp. 29-34

Abstract

These studies describe the effects of recombinant human (rHu) and recombinant murine (rMu) interleukin 12 (IL-12) in the porcine system. Toexamine the effects of rHu and rMu IL-12, porcine PBL were cultured with rHu and rMu IL-12, and then used for a NK assay. Significant enhancement of porcine NK cell cytotoxicity was observed, and maximal enhancement was reached after 26 hr culture in nanogram quantities (5 ng/ml= 10 units/ml) of rHu and rMu IL-12. Monoclonal antibody (mAb) specific for human IL-12, C8.6, blocked rHu IL-12 enhanced porcine natural killer (NI) cytotoxicity. It was also shown that rHu IL-12 had additiveeffects on enhancement of Mt cell cytotoxicity with PNK-E/G7 mAbs which react with cytolytic trigger molecules on porcine NK cells. By using purified NK cells, porcine tumor necrosis factor-alpha (TNF-alpha) secretion was measured on rHu IL-12 and PNK-E/G7 mAb-activated NR cells. rHu IL-12 and PNK-E/G7 mAbs synergize to induce TNP-alpha secretion. These data indicate that rHu and rMu IL-12 are cross-reactive in the porcine system and suggest the potential use of the porcine system as an important animal model for further study of rHu IL-12. (C) 1996 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 06:11:30