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Titolo:
MALARIA DIAGNOSIS - STANDARDIZATION OF A POLYMERASE CHAIN-REACTION FOR THE DETECTION OF PLASMODIUM-FALCIPARUM PARASITES IN INDIVIDUALS WITHLOW-GRADE PARASITEMIA
Autore:
ZALIS MG; FERREIRADACRUZ MF; BALTHAZARGUEDES HC; BANIC DM; ALECRIM W; SOUZA JM; DRUILHE P; DANIELRIBEIRO CT;
Indirizzi:
FIOCRUZ MS,INST OSWALDO CRUZ,DEPT IMMUNOL,WHO,COLLABORATING CTR RES &TRAINING IMMUNOL PARA BR-21045900 RIO JANEIRO BRAZIL FIOCRUZ MS,INST OSWALDO CRUZ,DEPT IMMUNOL,WHO,COLLABORATING CTR RES &TRAINING IMMUNOL PARA BR-21045900 RIO JANEIRO BRAZIL FED UNIV RIO DE JANEIRO,DEPT BIOPHYS BR-21941 RIO JANEIRO BRAZIL INST TROP MED MANAUS AMAZONAS BRAZIL INST EVANDRO CHAGAS BELEM PARA BRAZIL INST PASTEUR PARIS FRANCE
Titolo Testata:
Parasitology research
fascicolo: 7, volume: 82, anno: 1996,
pagine: 612 - 616
SICI:
0932-0113(1996)82:7<612:MD-SOA>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE; AMPLIFICATION; CULTURE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
14
Recensione:
Indirizzi per estratti:
Citazione:
M.G. Zalis et al., "MALARIA DIAGNOSIS - STANDARDIZATION OF A POLYMERASE CHAIN-REACTION FOR THE DETECTION OF PLASMODIUM-FALCIPARUM PARASITES IN INDIVIDUALS WITHLOW-GRADE PARASITEMIA", Parasitology research, 82(7), 1996, pp. 612-616

Abstract

In Brazil, no study has been done concerning the detection of malariaparasites by polymerase chain reaction (PCR) related to the diagnosisof Plasmodium falciparum malaria. In the present report we describe ahighly sensitive methodology for malaria diagnosis using a nested PCRmethod based on amplification of the p126 P. falciparum gene detectedby simple ethidium bromide staining. The P. falciparum Pale Alto strain (culture samples) was serially diluted in blood from an uninfected donor to a final level of parasitemia corresponding to 10(-8)% and wasprocessed for PCR amplification. In each of these dilutions a parasitological examination was performed to compare the sensitivity with that of PCR amplification. Blood samples (field samples) were obtained from 51 malarious patients with positive thick blood smears (TBS) who were living in endemic regions of the Brazilian Amazon. They corresponded to 42 P. falciparum and 9 P. vivax cases, with parasitemia levels ranging from only 16 to 20,200 parasites/mu l for P. falciparum disease and from 114 to 11,000 parasites/mu l for P. vivax malaria. We demonstrate that the use of nested PCR allows the detection of 0.005 parasites/mu l without the use of radioactive material. The use of a 1-ml sample volume and the organic DNA extraction method should be suitable in blood banks and for the evaluation of patients during and after drug treatment.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 05:05:23