Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
DIMETHYLALLYL DIPHOSPHATE - KAEMPFEROL 8-DIMETHYLALLYL TRANSFERASE INEPIMEDIUM-DIPHYLLUM CELL-SUSPENSION CULTURES
Autore:
YAMAMOTO H; KIMATA J; SENDA M; INOUE K;
Indirizzi:
GIFU PHARMACEUT UNIV,DEPT PHARMACOGNOSY,6-1 MITAHORA HIGASHI 5 CHOME GIFU 502 JAPAN
Titolo Testata:
Phytochemistry
fascicolo: 1, volume: 44, anno: 1997,
pagine: 23 - 28
SICI:
0031-9422(1997)44:1<23:DD-K8T>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENZYMATIC PRENYLATION; BIOSYNTHESIS;
Keywords:
EPIMEDIUM DIPHYLLUM; BERBERIDACEAE; DIMETHYLALLYL TRANSFERASE; CELL SUSPENSION CULTURES; BIOSYNTHESIS; DES-O-METHYLANHYDROICARITIN; KAEMPFEROL; EPIMEDOSIDE A;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
15
Recensione:
Indirizzi per estratti:
Citazione:
H. Yamamoto et al., "DIMETHYLALLYL DIPHOSPHATE - KAEMPFEROL 8-DIMETHYLALLYL TRANSFERASE INEPIMEDIUM-DIPHYLLUM CELL-SUSPENSION CULTURES", Phytochemistry, 44(1), 1997, pp. 23-28

Abstract

The enzymatic formation of des-O-methylanhydroicaritin from dimethylallyl diphosphate and kaempferol was investigated in the cell-free extract of Epimedium diphyllum cell suspension cultures. The enzyme catalysing the dimethylallyl group transfer to C-8 position of kaempferol (EC 2.5.1.) was membrane-bound and required divalent cations such as Mg2 and Mn2+. Mg2+ was the most effective for the reaction. The enzyme showed a very broad pH optimum in the alkaline region, pH 7.5-11.0. It required dimethylallyl diphosphate as a sole prenyl donor, but had a rather broad substrate specificity for the prenyl acceptor. Not only kaempferol, but also quercetin (39%), apigenin (60%) and luteolin (34%) were prenylated, whereas kaempferol glycosides, naringenin and genistein were not prenylated. These results suggested that the prenylation reaction of kaempferol precedes the glycosylation step in epimedoside Abiosynthesis. The apparent K-m values for dimethylallyl diphosphate and kaempferol were 0.58 and 0.13 mM, respectively. Copyright (C) 1996 Elsevier Science Ltd

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 03:15:12