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Titolo:
CONCANAVALIN-A ENHANCES QUISQUALATE-INDUCED CALCIUM INFLUX IN CEREBELLAR GRANULE CELLS IN CULTURE
Autore:
DANYSZ W; RAULLI R; WROBLEWSKI JT;
Indirizzi:
MERCK & CO INC,DEPT PHARMACOL,100-104 ECKENHEIMER LANDSTR D-60318 FRANKFURT GERMANY AMER RED CROSS,DEPT CELL BIOL ROCKVILLE MD 20855 GEORGETOWN UNIV,SCH MED,DEPT PHARMACOL WASHINGTON DC 20007
Titolo Testata:
Acta Neurobiologiae Experimentalis
fascicolo: 3, volume: 56, anno: 1996,
pagine: 649 - 656
SICI:
0065-1400(1996)56:3<649:CEQCII>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
STIMULATED PHOSPHOINOSITIDE HYDROLYSIS; GLUTAMATE RECEPTORS; KAINIC ACID; RAT-BRAIN; HIPPOCAMPAL-NEURONS; SINGLE RECEPTOR; AMPA BINDING; DESENSITIZATION; MODULATION; CYCLOTHIAZIDE;
Keywords:
CONCANAVALIN A; AMPA RECEPTORS; GLUTAMATE; QUISQUALATE; KAINATE; CA-45(2+) INFLUX; CEREBELLAR GRANULE CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
W. Danysz et al., "CONCANAVALIN-A ENHANCES QUISQUALATE-INDUCED CALCIUM INFLUX IN CEREBELLAR GRANULE CELLS IN CULTURE", Acta Neurobiologiae Experimentalis, 56(3), 1996, pp. 649-656

Abstract

In primary cultures of cerebellar granule cells kainate Ca-45(2+), partially sensitive to the produced marked influx of N-methyl-D-aspartate (NMDA) antagonist, (+/-)-2-carboxypiperazin-4-yl)-propyl-1-phosphonic acid (CPP), indicating involvement of an NMDA receptor-sensitive component that may be secondary to kainate-induced glutamate release. Sodium removal partially inhibited kainate's effect. Quisqualate also produced influx of Ca-45(2+), but with lower efficacy and higher potency than Ca kainate. This action of quisqualate was unaffected by CPP and by sodium removal. Preincubation of cells with the plant lectin concanavalin A (Con A), but not with its succinyl derivative, enhanced quisqualate- induced calcium influx, and to a lesser extent kainate's effect. Inclusion of quisqualate in preincubation medium antagonized Con A potentiation of quisqualate response. Also Con A was ineffective when included in the incubation medium only, without preincubation. Preincubation of rat brain cortical membranes with Con A but not with succinyl Con A increased the binding of the AMPA receptor agonist, lpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid ([H-3]AMPA). The resultssuggest that Con A enhancement of quisqualate response possibly involves the modification of an AMPA recognition site and requires preincubation in the absence of an agonist (here quisqualate).

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Documento generato il 03/04/20 alle ore 07:59:13