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Titolo:
CHRONIC MORPHINE ADMINISTRATION ENHANCES THE EXPRESSION OF KV1.5 AND KV1.6 VOLTAGE-GATED K-CORD( CHANNELS IN RAT SPINAL)
Autore:
MATUSLEIBOVITCH N; VOGEL Z; EZRAMACABEE V; ETKIN S; NEVO I; ATTALI B;
Indirizzi:
WEIZMANN INST SCI,DEPT NEUROBIOL IL-76100 REHOVOT ISRAEL WEIZMANN INST SCI,DEPT NEUROBIOL IL-76100 REHOVOT ISRAEL
Titolo Testata:
Molecular brain research
fascicolo: 2, volume: 40, anno: 1996,
pagine: 261 - 270
SICI:
0169-328X(1996)40:2<261:CMAETE>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
ROOT GANGLION COCULTURES; MESSENGER-RNA EXPRESSION; OPIATE EXPOSURE LEADS; IN-SITU HYBRIDIZATION; NEURON HYBRID-CELLS; OPIOID-RECEPTOR; LOCUS-COERULEUS; POTASSIUM CONDUCTANCE; ADENYLATE-CYCLASE; FUNCTIONAL EXPRESSION;
Keywords:
OPIOID RECEPTOR; OPIATE TOLERANCE; HYBRIDIZATION; IN-SITU; SPINAL CORD; K+ CHANNEL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
72
Recensione:
Indirizzi per estratti:
Citazione:
N. Matusleibovitch et al., "CHRONIC MORPHINE ADMINISTRATION ENHANCES THE EXPRESSION OF KV1.5 AND KV1.6 VOLTAGE-GATED K-CORD( CHANNELS IN RAT SPINAL)", Molecular brain research, 40(2), 1996, pp. 261-270

Abstract

Prolonged opiate administration leads re, the development of tolerance and dependence. These phenomena are accompanied by selective regulation of distinct cellular proteins and mRNAs, including ionic channels. Acute opiate administration differentially affects voltage-dependent K+ currents. Whereas, opiate activation of K+ channels is well established opioid-induced inhibition of K+ conductance has also been studied. In this study, we focused on the effect of chronic morphine exposureon voltage-dependent Shaker-related Kv1.5 and Kv1.6 K+ channel gene expression and on Kv1.5 protein levels in the rat spinal cord. Several experimental approaches including in-situ hybridization, RNAse protection, reverse transcriptase-polymerase chain reaction (RT-PCR), Westernblotting and immunohistochemistry were employed. We found that motor neurons are highly enriched in Kv1.5 and Kv1.6 mRNA and in Kv1.5 channel protein. Moreover, we found significant increases in the amount of mRNA encoding for these two K+ channels and in Kv1.5 channel protein in the spinal cord of morphine-treated rats, compared with controls. For example, quantitative in-situ hybridization, revealed a 2.1 +/- 0.15- and 2.3 +/- 0.5-fold increase in Kv1.5 and Kv1.6 channel mRNA levels, respectively. Similar results were obtained by semiquantitative RT-PCR analyses. Kv1.5 protein level was increased by 1.9-fold in the spinal cord of morphine-treated rats. Our results suggest that Kv1.5 and Kv1.6 Shaker K+ channels play an important role in regulating motor activity and that increases in mRNA and protein levels of the spinal cordK+ channels after chronic morphine exposure could be viewed as a cellular adaptation which compensates for a persistent opioid-induced inhibition of K+ channel activity. These alterations may account, in part,for the cellular events leading to opiate tolerance and dependence.

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Documento generato il 03/07/20 alle ore 01:16:45