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Titolo:
DETERMINATION OF CLOZAPINE AND ITS 2 MAJOR METABOLITES IN HUMAN SERUMBY LIQUID-CHROMATOGRAPHY USING ULTRAVIOLET DETECTION
Autore:
HARIHARAN U; HARIHARAN M; NAICKAR JS; TANDON R;
Indirizzi:
UNIV MICHIGAN,SCH MED,DEPT PSYCHIAT,1150 W MED CTR DR,MSRB-1 ANN ARBOR MI 48109 UNIV MICHIGAN,COLL PHARM,DEPT PSYCHIAT ANN ARBOR MI 48109
Titolo Testata:
Journal of liquid chromatography & related technologies
fascicolo: 15, volume: 19, anno: 1996,
pagine: 2409 - 2417
SICI:
1082-6076(1996)19:15<2409:DOCAI2>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
NORCLOZAPINE; PLASMA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
8
Recensione:
Indirizzi per estratti:
Citazione:
U. Hariharan et al., "DETERMINATION OF CLOZAPINE AND ITS 2 MAJOR METABOLITES IN HUMAN SERUMBY LIQUID-CHROMATOGRAPHY USING ULTRAVIOLET DETECTION", Journal of liquid chromatography & related technologies, 19(15), 1996, pp. 2409-2417

Abstract

A new, simple, reverse phase, and highly reproducible HPLC-UV method that has all the comprehensive features of good column, mobile phase, internal standard and extraction has been developed for the assay of clozapine (CLZ), norclozapine (NCLZ) and clozapine-N-oxide (CLZNO) in human serum. The method is very easy to adapt, overcomes the problems of earlier methods and is very economical. Amoxapine is the internal standard. All three analytes are extracted from alkaline serum using ethyl acetate and the absolute extraction efficiency is similar to 95%. The UV detector is set at 230 nm. This mobile phase is a mixture of phosphate buffer (0.05M, pH 2.7), acetonitrile and methanol (62:20:18 v/v) and contains 2.5 mt triethylamine per liter of solution. The detection limit of the method is 2 ng/mL for CLZ and NCLZ and 4 ng/mL for CLZNO. The mean CV for the method is 5%.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/01/20 alle ore 16:51:56