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Titolo:
DISTRIBUTION OF GAP-43 MESSENGER-RNA IN THE IMMATURE AND ADULT CEREBELLUM - A ROLE FOR GAP-43 IN CEREBELLAR DEVELOPMENT AND NEUROPLASTICITY
Autore:
CONSOLEBRAM LM; FITZPATRICKMCELLIGOTT SG; MCELLIGOTT JG;
Indirizzi:
TEMPLE UNIV,SCH MED,DEPT PHARMACOL,3420 N BROAD ST PHILADELPHIA PA 19140 TEMPLE UNIV,SCH MED,DEPT PHARMACOL PHILADELPHIA PA 19140 DUPONT MERCK PHARMACEUT CO,CENT RES & MED PROD WILMINGTON DE 19880
Titolo Testata:
Developmental brain research
fascicolo: 1, volume: 95, anno: 1996,
pagine: 97 - 106
SICI:
0165-3806(1996)95:1<97:DOGMIT>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
KINASE-C SUBSTRATE; GROWTH-ASSOCIATED PROTEIN; VESTIBULOOCULAR REFLEX ADAPTATION; AXONALLY TRANSPORTED PROTEINS; CALMODULIN-BINDING-PROTEIN; RAT-BRAIN; MESSENGER-RNA; INSITU HYBRIDIZATION; NERVE GROWTH; NITRIC-OXIDE;
Keywords:
GAP-43 MESSENGER-RNA; CEREBELLUM; INFERIOR OLIVE; IN SITU HYBRIDIZATION; SYNAPTOGENESIS; AXONAL OUTGROWTH; NEUROPLASTICITY; CEREBELLAR GRANULE CELL; CEREBELLAR NUCLEUS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
72
Recensione:
Indirizzi per estratti:
Citazione:
L.M. Consolebram et al., "DISTRIBUTION OF GAP-43 MESSENGER-RNA IN THE IMMATURE AND ADULT CEREBELLUM - A ROLE FOR GAP-43 IN CEREBELLAR DEVELOPMENT AND NEUROPLASTICITY", Developmental brain research, 95(1), 1996, pp. 97-106

Abstract

Expression of GAP-43 mRNA in the rat cerebellum and inferior olivary nucleus was examined at birth, during postnatal development and in theadult by both Northern and in situ hybridization. Northern blot analysis revealed that cerebellar GAP-43 mRNA expression increases from birth to postnatal day (PD) 7 and then declines to a lower level the adult. At birth, in situ hybridization experiments showed intense labelingof GAP-43 mRNA in the premigratory, but not the germinal, zone of thecerebellar external granule cell layer. Localization of GAP-43 withinthe premigratory zone, a layer containing post-mitotic granule cells,indicates that granule cells begin expressing GAP-43 mRNA after finalmitosis and during axonal outgrowth of the parallel fibers. The deep cerebellar nuclei and the inferior olive were also intensely labeled at birth. GAP-43 mRNA was localized in granule cells during their migration through the molecular layer of the developing cerebellum and after their arrival in the internal granule cell layer. By PD 21, the pattern of GAP-43 expression was similar to that observed in the adult; GAP-43 mRNA was localized to the internal granule layer and the inferiorolive with minimal to no hybridization in the deep cerebellar nuclei and none in the molecular layer. Purkinje cells were devoid of GAP-43 mRNA throughout the postnatal and adult periods. In light of our observations, we propose that GAP-43 is a critical factor in granule cell differentiation/migration, as well as in parallel and climbing fiber axonal outgrowth and synaptogenesis during development. Localization of GAP-43 mRNA within granule and inferior olivary cells of adult animalsindicates that GAP-43 protein observed in the molecular layer is transported from these cells to their terminals in the molecular layer suggesting that GAP-43 is also an intrinsic presynaptic determinant in cerebellar neuroplasticity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 11:29:05