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Titolo:
THE DUAL PEPTIDASE INHIBITOR RB101 INDUCES A LONG-LASTING INCREASE INTHE EXTRACELLULAR LEVEL OF MET-ENKEPHALIN-LIKE MATERIAL IN THE NUCLEUS-ACCUMBENS OF FREELY MOVING RATS
Autore:
DAUGE V; MAUBORGNE A; CESSELIN F; FOURNIEZALUSKI MC; ROQUES BP;
Indirizzi:
UNIV PARIS 05,FAC PHARM,DEPT PHARMACOCHIM MOL & STRUCT,INSERM,U266,URA D 1500 CNRS,4 AVE OBSERV F-75270 PARIS 06 FRANCE UNIV PARIS 05,FAC PHARM,DEPT PHARMACOCHIM MOL & STRUCT,INSERM,U266,URA D 1500 CNRS F-75270 PARIS 06 FRANCE CHU PITIE SALPETRIERE,DEPT NEUROBIOL CELLULAR & FONCT,INSERM,U288 PARIS FRANCE
Titolo Testata:
Journal of neurochemistry
fascicolo: 3, volume: 67, anno: 1996,
pagine: 1301 - 1308
SICI:
0022-3042(1996)67:3<1301:TDPIRI>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
VENTRAL TEGMENTAL AREA; METHIONINE-ENKEPHALIN; DEGRADING ENZYMES; ENDOGENOUS ENKEPHALINS; OPIOID RECEPTORS; SPINAL-CORD; BRAIN; INVIVO; DOPAMINE; DELTA;
Keywords:
MICRODIALYSIS; MET-ENKEPHALIN-LIKE MATERIAL; NUCLEUS ACCUMBENS; DUAL PEPTIDASE INHIBITOR; MOTOR ACTIVITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
V. Dauge et al., "THE DUAL PEPTIDASE INHIBITOR RB101 INDUCES A LONG-LASTING INCREASE INTHE EXTRACELLULAR LEVEL OF MET-ENKEPHALIN-LIKE MATERIAL IN THE NUCLEUS-ACCUMBENS OF FREELY MOVING RATS", Journal of neurochemistry, 67(3), 1996, pp. 1301-1308

Abstract

This study was carried out to analyze the extracellular levels of Met-enkephalin-like material in the nucleus accumbens, a brain structure involved in the effects of opioids on motor activity and reward processes, using microdialysis in awake and freely moving rats, combined with a sensitive radioimmunoassay, The levels of Met-enkephalin-like material were measured after administration of a dual inhibitor of enkephalin-degrading enzymes, RB101, to evaluate its in vivo protecting effects. The basal levels of Met-enkephalin-like immunoreactivity in the nucleus accumbens were similar to 1.2 pg/30 min or 2.2 fmol/30 min (37 pM), Perfusion of KCl (100 mM) produced a 17-fold increase in the levelof Met-enkephalin-like material in this structure. During the 8-h perfusion, which started at 9 a.m., a spontaneous increase of the basal level of Met-enkephalin-like material in the nucleus accumbens occurredbetween 4 and 4:30 p.m., suggesting the existence of variation in opioid peptide secretion, at least in this structure. Intraperitoneal injection of RB101 induced a dose-dependent and long-lasting (210-min) increase in the extracellular levels of Met-enkephalin-like material, A prolonged effect was also observed in the behavioral studies in which the inhibitor increased global motor activity of rats 210 min after injection, These data represent the first direct evidence that dual inhibitors of enkephalin-degrading enzymes increase in vivo the extracellular levels of Met-enkephalin-like material in awake and freely moving rats.

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Documento generato il 07/07/20 alle ore 14:56:51