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Titolo:
CULTURED LEPTOMENINGEAL CELLS SECRETE CEREBROSPINAL-FLUID PROTEINS
Autore:
OHE Y; ISHIKAWA K; ITOH Z; TATEMOTO K;
Indirizzi:
GUNMA UNIV,INST MOL & CELLULAR REGULAT,DEPT MOL PHYSIOL,SHOWA MACHI MAEBASHI GUMMA 371 JAPAN GUNMA UNIV,INST MOL & CELLULAR REGULAT,DEPT MOL PHYSIOL MAEBASHI GUMMA 371 JAPAN GUNMA UNIV,INST MOL & CELLULAR REGULAT,GASTROINTESTINAL RES LAB MAEBASHI GUMMA 371 JAPAN
Titolo Testata:
Journal of neurochemistry
fascicolo: 3, volume: 67, anno: 1996,
pagine: 964 - 971
SICI:
0022-3042(1996)67:3<964:CLCSCP>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
FACTOR-BINDING-PROTEIN; GROWTH FACTOR-II; PROSTAGLANDIN-D SYNTHETASE; CENTRAL-NERVOUS-SYSTEM; RAT-BRAIN; MESSENGER-RNA; APOLIPOPROTEIN-E; CHOROID-PLEXUS; NUCLEOTIDE-SEQUENCE; TRANSFERRIN GENE;
Keywords:
BETA-TRACE PROTEIN; PIA MATER; ARACHNOID; TRANSFERRIN; APOLIPOPROTEIN E; CYSTATIN C; BETA-2-MICROGLOBULIN; CULTURE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
58
Recensione:
Indirizzi per estratti:
Citazione:
Y. Ohe et al., "CULTURED LEPTOMENINGEAL CELLS SECRETE CEREBROSPINAL-FLUID PROTEINS", Journal of neurochemistry, 67(3), 1996, pp. 964-971

Abstract

To extrapolate the function of the leptomeninges, we examined the profile of the proteins secreted from the cultured leptomeningeal cells prepared from 1-2-day-old rats. In sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of the medium conditioned with the cultured cells, 20-25 differentially distinctive protein bands were noted. Through several chromatographic procedures (Sephadex G-75, Mono Q, and 7C(8)-300), altogether 18 proteins were purified to homogeneity, andthe partial amino acid sequence of each protein was determined, Homology search revealed that the major proteins included prostaglandin-D-synthase or beta-trace protein, insulin-like growth factor (IGF)-II, IGF-binding protein-2, apolipoprotein E, beta 2-microglobulin, cystatin C, transferrin, peptidyl-prolyl cis-trans isomerase or cyclophilin C, secreted protein acidic and rich in cysteine, ubiquitin, lysozyme C, extracellular superoxide dismutase, and collagen alpha-1 (III), Most ofthese proteins are known to be the major brain-derived protein constituents of CSF and are thought to play important roles in certain biological events in the brain, Considering the morphological features, thepresent findings suggest the importance of the leptomeninges as an origin of such proteins in CSF.

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Documento generato il 03/04/20 alle ore 20:30:04