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Titolo:
THE FUNCTION OF INTERMEDIATE FILAMENTS IN CELL-SHAPE AND CYTOSKELETALINTEGRITY
Autore:
GOLDMAN RD; KHUON S; CHOU YH; OPAL P; STEINERT PM;
Indirizzi:
NORTHWESTERN UNIV,SCH MED,DEPT CELL & MOL BIOL,303 E CHICAGO AVE CHICAGO IL 60611 NIAMSD,SKIN BIOL LAB,NIH BETHESDA MD 20892
Titolo Testata:
The Journal of cell biology
fascicolo: 4, volume: 134, anno: 1996,
pagine: 971 - 983
SICI:
0021-9525(1996)134:4<971:TFOIFI>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPIDERMOLYSIS-BULLOSA SIMPLEX; MICROTUBULE-ASSOCIATED PROTEINS; CULTURED FIBROBLASTS; CYTOPLASMIC FIBERS; SMOOTH-MUSCLE; IN-VIVO; VIMENTIN; KERATIN; SEQUENCE; NETWORKS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
76
Recensione:
Indirizzi per estratti:
Citazione:
R.D. Goldman et al., "THE FUNCTION OF INTERMEDIATE FILAMENTS IN CELL-SHAPE AND CYTOSKELETALINTEGRITY", The Journal of cell biology, 134(4), 1996, pp. 971-983

Abstract

This study describes the development and use of a specific method fordisassembling intermediate filament (IF) networks in living cells. Ittakes advantage of the disruptive effects of mimetic peptides derivedfrom the amino acid sequence of the helix initiation 1A domain of IF protein chains. The results demonstrate that at 1:1 molar ratios, these peptides disassemble vimentin IF into small oligomeric complexes andmonomers within 30 min at room temperature in vitro. Upon microinjection into cultured fibroblasts, these same peptides induce the rapid disassembly of IF networks. The disassembly process is accompanied by a dramatic alteration in cell shape and the destabilization of microtubule and actin-stress fiber networks. These changes in cell shape and IFassembly states are reversible. The results are discussed with respect to the roles of IF in cell shape and the maintenance of the integrity and mechanical properties of the cytoplasm, as well as the stabilityof the other major cytoskeletal systems.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 20:43:44