Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
CLONING AND EXPRESSION IN YEAST PICHIA-PASTORIS OF A BIOLOGICALLY-ACTIVE FORM OF CYN-D-1, THE MAJOR ALLERGEN OF BERMUDA GRASS-POLLEN
Autore:
SMITH PM; SUPHIOGLU C; GRIFFITH IJ; THERIAULT K; KNOX RB; SINGH MB;
Indirizzi:
UNIV MELBOURNE,SCH BOT PARKVILLE VIC 3052 AUSTRALIA UNIV MELBOURNE,SCH BOT PARKVILLE VIC 3052 AUSTRALIA IMMULOG PHARMACEUT CORP WALTHAM MA 00000
Titolo Testata:
Journal of allergy and clinical immunology
fascicolo: 2, volume: 98, anno: 1996,
pagine: 331 - 343
SICI:
0091-6749(1996)98:2<331:CAEIYP>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYNODON-DACTYLON POLLEN; ALCOHOL OXIDASE GENES; AMB-A-II; LOL-P-I; RYE-GRASS; METHYLOTROPHIC YEAST; IMMUNOCHEMICAL CHARACTERIZATION; MONOCLONAL-ANTIBODIES; CROSS-ALLERGENICITY; SIGNAL SEQUENCES;
Keywords:
CYN D 1; BERMUDA GRASS; POLLEN ALLERGENS; CDNA CLONING; EXPRESSION 1; YEAST; IGE BINDING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
P.M. Smith et al., "CLONING AND EXPRESSION IN YEAST PICHIA-PASTORIS OF A BIOLOGICALLY-ACTIVE FORM OF CYN-D-1, THE MAJOR ALLERGEN OF BERMUDA GRASS-POLLEN", Journal of allergy and clinical immunology, 98(2), 1996, pp. 331-343

Abstract

Background: Pollen of grasses, such as Bermuda grass (Cynodon dactylon), represent a major cause of type I allergy. Objective: In this report we attempted to clone and express a biologically active from of recombinant Cyn d 1, the major allergen of Bermuda grass pollen, in the yeast Pichia pastoris. Methods: Clones encoding Cyn d 1 were isolated by screening a Bermuda grass pollen complementary DNA library with specific monoclonal antibodies and by polymerase chain reaction amplification. Recombinant Cyn d 1 was expressed in Escherichia coli and yeast. The expressed proteins were analyzed by Western blotting to assess binding to Cyn d 1-specific monoclonal antibodies and IgE from sera of patients allergic to Bermuda grass pollen. Results: Two isoforms of Cyn d 1 were cloned. Recombinant Cyn d 1 expressed in bacteria bound two monoclonal antibodies raised against Cyn d 1 but was not recognized by IgE from sera of patients allergic to Bermuda grass pollen. Cyn d 1 expressed in yeast bound both the monoclonal antibodies and human IgE. Conclusion: an IgE-reactive Cyn d 1 was expressed in yeast but not in bacteria, suggesting that posttranslational modifications (e.g., glycosylation), which occur in eukaryotic cells such as yeast, are necessaryfor the production of a biologically active allergen.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 13:46:20