Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
MATERIAL-INDUCED UP-REGULATION OF LEUKOCYTE CD11B DURING WHOLE-BLOOD CONTACT - MATERIAL DIFFERENCES AND A ROLE FOR COMPLEMENT
Autore:
GEMMELL CH; BLACK JP; YEO EL; SEFTON MV;
Indirizzi:
TORONTO HOSP,67 COLL ST,ROOM 406 TORONTO ON M5G 2M1 CANADA UNIV TORONTO,DEPT MED TORONTO ON CANADA UNIV TORONTO,DEPT CHEM ENGN & APPL CHEM TORONTO ON CANADA
Titolo Testata:
Journal of biomedical materials research
fascicolo: 1, volume: 32, anno: 1996,
pagine: 29 - 35
SICI:
0021-9304(1996)32:1<29:MUOLCD>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
SYNTHETIC VASCULAR PROSTHESES; CARDIOPULMONARY BYPASS; CHEMOTACTIC FACTORS; ADHESION MOLECULES; ALTERNATIVE PATHWAY; CELL-SURFACE; L-SELECTIN; ACTIVATION; HEMODIALYSIS; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
C.H. Gemmell et al., "MATERIAL-INDUCED UP-REGULATION OF LEUKOCYTE CD11B DURING WHOLE-BLOOD CONTACT - MATERIAL DIFFERENCES AND A ROLE FOR COMPLEMENT", Journal of biomedical materials research, 32(1), 1996, pp. 29-35

Abstract

Material-induced thrombogenicity is in part a consequence of leukocyte activation. To evaluate and compare material-induced platelet damage, we have expanded our in vitro flow cytometric immunoassay to includeassessment of leukocyte activation. We have used a very simple systemwhereby fresh, heparinized whole blood contacts materials for 1 h at 37 degrees C under low shear. Unlike other tests that focus on adherent leukocytes, this assay evaluates the leukocytes in the whole blood drained from the tube (1.57 mm internal diameter, 25 cm length) after material contact. We demonstrate that whole blood contact with a polyvinyl alcohol (PVA) hydrogel surface leads to a twofold up-regulation inCD11b surface expression of all monocytes and neutrophils. The activation is metal-ion dependent and highly material dependent in that blood contact with polyethylene and Silastic(TM) surfaces leads to minimalactivation. The shedding of L-selection as a marker of leukocyte activation was found to be unsuitable in our assay given its ease of shedding in resting heparinized whole blood. Further, plasma levels of complement components Bb and sC5b-9 (ELISA assays) were significantly elevated only after blood contact with PVA hydrogel surfaces (9.4 mu g/mL sC5b-9 and 9.6 mu g/mL Bb). Use of recombinant soluble human CR1 (sCR1) to inhibit the action or the C3 and C5 convertases completely inhibited sC5b-9 levels in whole blood after contact with PVA hydrogel surfaces and inhibited CD11b up-regulation by over 70%, suggesting that material-induced leukocyte activation is partially mediated by C5a production. (C) 1996 John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/21 alle ore 03:44:11