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Titolo:
AN APPROACH FOR FLUOROMETRIC-DETERMINATION OF GLYCOSYLTRANSFERASE ACTIVITIES
Autore:
PALMERINI CA; DATTI A; VANDERELST IE; MINUTI L; ORLACCHIO A;
Indirizzi:
UNIV PERUGIA,DIPARTIMENTO BIOL CELLULARE & MOL,SEZ BIOCHIM & BIOL MOLI-06126 PERUGIA ITALY UNIV PERUGIA,DIPARTIMENTO BIOL CELLULARE & MOL,SEZ BIOCHIM & BIOL MOLI-06126 PERUGIA ITALY UNIV PERUGIA,DIPARTIMENTO CHIM I-06126 PERUGIA ITALY
Titolo Testata:
Glycoconjugate journal
fascicolo: 4, volume: 13, anno: 1996,
pagine: 631 - 636
SICI:
0282-0080(1996)13:4<631:AAFFOG>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
N-ACETYLGLUCOSAMINYLTRANSFERASE-III; GLYCOSYLATION; OLIGOSACCHARIDES; PURIFICATION; ELONGATION; TISSUES; GLCNAC; TUMOR; ASSAY; CELLS;
Keywords:
GLYCOSYLTRANSFERASES; ASSAYS; FLUORESCENCE; DANSYL CHLORIDE; BETA-1,6 N-ACETYLGLUCOSAMINYLTRANSFERASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
C.A. Palmerini et al., "AN APPROACH FOR FLUOROMETRIC-DETERMINATION OF GLYCOSYLTRANSFERASE ACTIVITIES", Glycoconjugate journal, 13(4), 1996, pp. 631-636

Abstract

A new strategy for the fluorometric determination of glycosyltransferase activities is reported. The method involves dansyl chloride derivatization of the reduced form (pNH(2)phenyl) of a hydrophobic, aglycon moiety covalently linked to a number of acceptor substrates (pNO(2)phenyl). Focusing on the Golgi enzyme core 2 N-acetylglucosaminyltransferase, we found that synthesis and fractionation of the dansylated substrate derivative were rapid, easy and inexpensive. Additionally, the corresponding enzyme assay proved reproducible and very sensitive, as 0.4 pmol of reaction product were readily detected. This fluorometric approach appears therefore to be a valid tool for investigating the monitoring differential expression of glycosyltransferases exhibiting low levels of enzyme activity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 08:49:30