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Titolo:
E-BOX SITES AND A PROXIMAL REGULATORY REGION OF THE MUSCLE CREATINE-KINASE GENE DIFFERENTIALLY REGULATE EXPRESSION IN DIVERSE SKELETAL-MUSCLES AND CARDIAC-MUSCLE OF TRANSGENIC MICE
Autore:
SHIELD MA; HAUGEN HS; CLEGG CH; HAUSCHKA SD;
Indirizzi:
UNIV WASHINGTON,DEPT BIOCHEM,BOX 357350 SEATTLE WA 98195 UNIV WASHINGTON,DEPT BIOCHEM SEATTLE WA 98195 BRISTOL MYERS SQUIBB PHARMACEUT RES INST SEATTLE WA 98121
Titolo Testata:
Molecular and cellular biology
fascicolo: 9, volume: 16, anno: 1996,
pagine: 5058 - 5068
SICI:
0270-7306(1996)16:9<5058:ESAAPR>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOOP-HELIX PROTEINS; HEAVY-CHAIN GENE; TROPONIN-C GENE; MOUSE MYOBLASTS; MDX MICE; ELEMENTS; BINDING; IDENTIFICATION; MULTIPLE; ENHANCER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
74
Recensione:
Indirizzi per estratti:
Citazione:
M.A. Shield et al., "E-BOX SITES AND A PROXIMAL REGULATORY REGION OF THE MUSCLE CREATINE-KINASE GENE DIFFERENTIALLY REGULATE EXPRESSION IN DIVERSE SKELETAL-MUSCLES AND CARDIAC-MUSCLE OF TRANSGENIC MICE", Molecular and cellular biology, 16(9), 1996, pp. 5058-5068

Abstract

Previous analysis of the muscle creatine kinase (MCK) gene indicated that control elements required for transcription in adult mouse musclediffered from those required in cell culture, suggesting that distinct modes of muscle gene regulation occur in vivo. To examine this further, we measured the activity of MCK transgenes containing E-box and promoter deletions in a variety of striated muscles. Simultaneous mutation of three E boxes in the 1,256-bp MCK 5' region, which abolished transcription in muscle cultures, had strikingly different effects in mice. The mutations abolished transgene expression in cardiac and tongue muscle and caused a reduction in expression in the soleus muscle (a muscle with many slow fibers) but did not affect expression in predominantly fast muscles: quadriceps, abdominals, and extensor digitorum longus. Other regulatory sequences with muscle-type-specific activities were found within the 358-bp 5'-flanking region. This proximal region conferred relatively strong expression in limb and abdominal skeletal muscles but was inactive in cardiac and tongue muscles. However, when the 206-bp 5' enhancer was ligated to the 358-bp region, high levels of tissue-specific expression were restored in all muscle types. These results indicate that E boxes and a proximal regulatory region are differentially required for maximal MCK transgene expression in different striated muscles. The overall results also imply that within skeletal muscles, the steady-state expression of the MCK gene and possibly othermuscle genes depends on transcriptional mechanisms that differ between fast and slow fibers as well as between the anatomical and physiological attributes of each specific muscle.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 19:18:43